The pH Signaling Transcription Factor PAC-3 Regulates Metabolic and Developmental Processes in Pathogenic Fungi

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dc.contributor.authorMartins, Maira Pompeu
dc.contributor.authorMartinez-Rossi, Nilce M.
dc.contributor.authorSanches, Pablo R.
dc.contributor.authorGomes, Eriston Vieira
dc.contributor.authorBertolini, Maria Celia [UNESP]
dc.contributor.authorPedersoli, Wellington R.
dc.contributor.authorSilva, Roberto Nascimento
dc.contributor.authorRossi, Antonio
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionMorgana Potrich Coll
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2019-10-04T12:41:20Z
dc.date.available2019-10-04T12:41:20Z
dc.date.issued2019-09-04
dc.description.abstractThe zinc finger transcription factor PAC-3/RIM101/PacC has a defined role in the secretion of enzymes and proteins in response to ambient pH, and also contributes to the virulence of species. Herein we evaluated the role of PAC-3 in the regulation of Neurospora crassa genes, in a model that examined the plant-fungi interactions. N. crassa is a model fungal species capable of exhibiting dynamic responses to its environment by employing endophytic or phytopathogenic behavior according to a given circumstance. Since plant growth and productivity are highly affected by pH and phosphorus (P) acquisition, we sought to verify the impact that induction of a Delta pac-3 mutation would have under limited and sufficient Pi availability, while ensuring that the targeted physiological adjustments mimicked ambient pH and nutritional conditions required for efficient fungal growth and development. Our results suggest direct regulatory functions for PAC-3 in cell wall biosynthesis, homeostasis, oxidation-reduction processes, hydrolase activity, transmembrane transport, and modulation of genes associated with fungal virulence. Pi-dependent modulation was observed mainly in genes encoding for transporter proteins or related to cell wall development, thereby advancing the current understanding regarding colonization and adaptation processes in response to challenging environments. We have also provided comprehensive evidence that suggests a role for PAC-3 as a global regulator in plant pathogenic fungi, thus presenting results that have the potential to be applied to various types of microbes, with diverse survival mechanisms.en
dc.description.affiliationUniv Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Ribeirao Preto, Brazil
dc.description.affiliationMorgana Potrich Coll, Dept Biofunct, Mineiros, Brazil
dc.description.affiliationSao Paulo State Univ, Inst Chem, Dept Biochem & Technol Chem, UNESP, Araraquara, Brazil
dc.description.affiliationUniv Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, Ribeirao Preto, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Inst Chem, Dept Biochem & Technol Chem, UNESP, Araraquara, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipBrazilian Funding Agency: Foundation for Support to Teaching, Research and Assistance - FAEPA of the HCFMRP-USP
dc.description.sponsorshipIdFAPESP: 2014/03847-7
dc.description.sponsorshipIdFAPESP: 2018/11319-1
dc.description.sponsorshipIdFAPESP: 2016/04274-6
dc.description.sponsorshipIdCNPq: 305252/2013-5
dc.description.sponsorshipIdCNPq: 304222/2013-5
dc.description.sponsorshipIdCAPES: 001
dc.format.extent11
dc.identifierhttp://dx.doi.org/10.3389/fmicb.2019.02076
dc.identifier.citationFrontiers In Microbiology. Lausanne: Frontiers Media Sa, v. 10, 11 p., 2019.
dc.identifier.doi10.3389/fmicb.2019.02076
dc.identifier.issn1664-302X
dc.identifier.lattes8817669953838863
dc.identifier.urihttp://hdl.handle.net/11449/186120
dc.identifier.wosWOS:000483753400001
dc.language.isoeng
dc.publisherFrontiers Media Sa
dc.relation.ispartofFrontiers In Microbiology
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectNeurospora crassa
dc.subjectmycorrhizal association
dc.subjectphytopathogen
dc.subjectinorganic orthophosphate
dc.subjectRNA-sequencing
dc.titleThe pH Signaling Transcription Factor PAC-3 Regulates Metabolic and Developmental Processes in Pathogenic Fungien
dc.typeArtigo
dcterms.rightsHolderFrontiers Media Sa
unesp.author.lattes8817669953838863
unesp.author.orcid0000-0003-1951-4741[8]

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