Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode

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dc.contributor.authorda Fonseca Alves, Rafael [UNESP]
dc.contributor.authorFranco, Diego Leoni
dc.contributor.authorCordeiro, Marli Tenório
dc.contributor.authorde Oliveira, Ernesto Marques
dc.contributor.authorFireman Dutra, Rosa Amalia
dc.contributor.authorDel Pilar Taboada Sotomayor, Maria [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.contributor.institutionCidade Universitária
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.date.accessioned2019-10-06T16:34:34Z
dc.date.available2019-10-06T16:34:34Z
dc.date.issued2019-10-01
dc.description.abstractThe Zika virus has recently emerged as a major worldwide threat because it induces microencephaly and other brain damage in babies born to infected mothers. Epidemiological monitoring of infection has been hampered by the lack of reliable tests capable of distinguishing between Zika and other Flavivirus infections, especially the dengue virus. The present work describes the electrochemical modification of pencil carbon graphite electrodes with a material derived from 3-amino-4-hydroxybenzoic acid. Solutions were prepared in acid medium and the material formed showed insulating behavior. Optical and electrical data suggested that carboxyl groups did not participate in the reaction mechanism. FT-IR spectra indicated that these groups were retained, enabling the proposal of a mechanism based on the data obtained. The material formed was used for immobilization of the ssDNAzv aminated oligonucleotide. Detection of the hybridization was based on the Δip resulting from the higher electrostatic repulsion of [Fe(CN)6]3−, caused by the increase of phosphates by addition of the complementary target sequence. The system showed a good linear correlation coefficient (r² = 0.997), with satisfactory selectivity and reproducibility, a low limit of detection (25.4 pM), and precision (RSD) of 3.1%, using real samples of human serum enriched with total extract of Zika virus RNA. Tests of the system using the total RNA extracted from dengue virus types 2 and 3 cultures showed little difference in the observed current, hence confirming the specificity of the system. These findings demonstrated the potential of the proposed biosensor.en
dc.description.affiliationDepartment of Analytical Chemistry Institute of Chemistry State University of São Paulo (UNESP)
dc.description.affiliationGrupo de Eletroquímica Aplicada a Polímeros e Sensores Instituto de Química Universidade Federal de Uberlândia
dc.description.affiliationOswaldo Cruz-Fiocruz Foundation Aggeu Magalhães Institute Cidade Universitária, Av. Prof. Moraes Rego s/nº, P.O. Box 7.472
dc.description.affiliationBiomedical Engineering Laboratory Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, Cidade Universitaria
dc.description.affiliationUnespDepartment of Analytical Chemistry Institute of Chemistry State University of São Paulo (UNESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 440605/2016-4
dc.identifierhttp://dx.doi.org/10.1016/j.snb.2019.126681
dc.identifier.citationSensors and Actuators, B: Chemical, v. 296.
dc.identifier.doi10.1016/j.snb.2019.126681
dc.identifier.issn0925-4005
dc.identifier.scopus2-s2.0-85067244178
dc.identifier.urihttp://hdl.handle.net/11449/189245
dc.language.isoeng
dc.relation.ispartofSensors and Actuators, B: Chemical
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subject3-Amino-4-hydroxybenzoic acid polymer
dc.subjectElectrochemical genosensor
dc.subjectPencil graphite
dc.subjectZika virus
dc.titleNovel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrodeen
dc.typeArtigo

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Artigos - Química Analítica - IQ