High concentrations of glucose reduce the oxidative metabolism of dog neutrophils in vitro

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dc.contributor.authorBosco, Anelise M. [UNESP]
dc.contributor.authorde Almeida, Breno F.M. [UNESP]
dc.contributor.authorPereira, Priscila P. [UNESP]
dc.contributor.authorNarciso, Luis G. [UNESP]
dc.contributor.authorLima, Valéria M.F. [UNESP]
dc.contributor.authorCiarlini, Paulo Cesar [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:28:21Z
dc.date.available2014-05-27T11:28:21Z
dc.date.issued2013-02-06
dc.description.abstractBackground: Dogs are commonly affected by hyperglycemic conditions. Hyperglycemia compromises the immune response and favors bacterial infections; however, reports on the effects of glucose on neutrophil oxidative metabolism and apoptosis are conflicting in humans and rare in dogs. Considering the many complex factors that affect neutrophil oxidative metabolism in vivo, we investigated in vitro the specific effect of high concentrations of glucose on superoxide production and apoptosis rate in neutrophils from healthy dogs.Results: The capacity of the neutrophils to reduce tetrazolium nitroblue decreased significantly in the higher concentration of glucose (15.13 ± 9.73% (8 mmol/L) versus 8.93 ± 5.71% (16 mmol/L)). However, there were no changes in tetrazolium nitroblue reduction at different glucose concentrations when the neutrophils were first activated with phorbol myristate acetate. High concentrations of glucose did not affect the viability and apoptosis rate of canine neutrophils either with or without prior camptothecin stimulation. This study provides the first evidence that high concentrations of glucose inhibit the oxidative metabolism of canine neutrophils in vitro in a manner similar to that which occurs in humans, and that the decrease in superoxide production did not increase the apoptosis rate.Conclusions: A high concentration of glucose reduces the oxidative metabolism of canine neutrophils in vitro. It is likely that glucose at high concentrations rapidly affects membrane receptors responsible for the activation of NADPH oxidase in neutrophils; therefore, the nonspecific immune response can be compromised in dogs with acute and chronic hyperglycemic conditions. © 2013 Bosco et al.; licensee BioMed Central Ltd.en
dc.description.affiliationDepartment of Clinical, Surgery and Animal Reproduction College of Veterinary Medicine of Araçatuba São Paulo State University, Araçatuba, SP
dc.description.affiliationUnespDepartment of Clinical, Surgery and Animal Reproduction College of Veterinary Medicine of Araçatuba São Paulo State University, Araçatuba, SP
dc.identifierhttp://dx.doi.org/10.1186/1746-6148-9-24
dc.identifier.citationBMC Veterinary Research, v. 9.
dc.identifier.doi10.1186/1746-6148-9-24
dc.identifier.file2-s2.0-84873243340.pdf
dc.identifier.issn1746-6148
dc.identifier.lattes3613940018299500
dc.identifier.orcid0000-0003-1480-5208
dc.identifier.scopus2-s2.0-84873243340
dc.identifier.urihttp://hdl.handle.net/11449/74562
dc.identifier.wosWOS:000314926200001
dc.language.isoeng
dc.relation.ispartofBMC Veterinary Research
dc.relation.ispartofjcr1.958
dc.relation.ispartofsjr0,934
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectHyperglycemia
dc.subjectLeukocyte dysfunction
dc.subjectProgrammed cell death
dc.subjectRespiratory burst
dc.subjectSuperoxide
dc.subjectBacteria (microorganisms)
dc.subjectCanis familiaris
dc.subjectsuperoxide
dc.subjectanimal
dc.subjectanimal disease
dc.subjectapoptosis
dc.subjectdog
dc.subjectdog disease
dc.subjectfemale
dc.subjecthyperglycemia
dc.subjectin vitro study
dc.subjectmale
dc.subjectmetabolism
dc.subjectneutrophil
dc.subjectoxidation reduction reaction
dc.subjectphysiology
dc.subjectAnimals
dc.subjectApoptosis
dc.subjectDog Diseases
dc.subjectDogs
dc.subjectFemale
dc.subjectMale
dc.subjectNeutrophils
dc.subjectOxidation-Reduction
dc.subjectSuperoxides
dc.titleHigh concentrations of glucose reduce the oxidative metabolism of dog neutrophils in vitroen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
unesp.author.lattes3613940018299500[6]
unesp.author.orcid0000-0003-1480-5208[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt

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Artigos - Clínica, Cirurgia e Reprodução Animal - FMVA