Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability

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dc.contributor.authorPellizzaro, Delise [UNESP]
dc.contributor.authorPolyzois, Gregory
dc.contributor.authorMachado, Ana Lucia [UNESP]
dc.contributor.authorGiampaolo, Eunice Teresinha [UNESP]
dc.contributor.authorSanitá, Paula Volpato [UNESP]
dc.contributor.authorVergani, Carlos Eduardo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Athens
dc.date.accessioned2014-05-27T11:26:57Z
dc.date.available2014-05-27T11:26:57Z
dc.date.issued2012-09-01
dc.description.abstractThe adhesion of Candida albicans to surfaces is the prerequisite for occurrence of denture stomatitis, a common disease diagnosed among denture wearers. A routine of denture cleansing is essential to prevent biofilm formation and the onset of this infection. The aim of this study was to investigate the effectiveness of combining brushing and cleansing agents in killing C. albicans biofilm. Disks of acrylic resin were made, sterilized, and inoculated with C. albicans (107 cfu/mL). After incubation (37°C/48 h), specimens were randomly assigned to 10 experimental groups (n=9): 5 subjected to brushing with distilled water or cleansing agents - dentifrice slurry, 2% chlorhexidine gluconate (CHX), 1% sodium hypochlorite (NaOCl), and Polident fresh cleanse® (combined method) - and 4 exposed to the cleansing agents without brushing (immersion). Non-cleansed specimens were used as positive controls. The viability of cells was evaluated by XTT reduction method. Results were analyzed by Mann-Whitney and Kruskal-Wallis tests (α=0.05). The combined method was significantly more effective (p<0.0001) in reducing biofilm viability than the immersion. Brushing with CHX and NaOCl resulted in 100% removal of the biofilm. Immersion in the agents reduced significantly (p<0.0001) the biofilm viability, with CHX being the most effective (p<0.0001). The use of the combined method of brushing with cleansing agents is an effective method to reduce C. albicans biofilm, being CHX and NaOCl the most effective solutions.en
dc.description.affiliationDepartment of Dental Materials and Prosthodontics Araraquara Dental School Universidade Estadual Paulista (UNESP), Araraquara, São Paulo
dc.description.affiliationDivision of Removable Prosthodontics Dental School University of Athens, Athens
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics Araraquara Dental School Universidade Estadual Paulista (UNESP), Araraquara, São Paulo
dc.format.extent547-554
dc.identifierhttp://dx.doi.org/10.1590/S0103-64402012000500013
dc.identifier.citationBrazilian Dental Journal, v. 23, n. 5, p. 547-554, 2012.
dc.identifier.doi10.1590/S0103-64402012000500013
dc.identifier.file2-s2.0-84872400694.pdf
dc.identifier.issn0103-6440
dc.identifier.issn1806-4760
dc.identifier.lattes8000248781842587
dc.identifier.lattes5737127334248549
dc.identifier.lattes3003130522427820
dc.identifier.orcid0000-0002-7375-4714
dc.identifier.scieloS0103-64402012000500013
dc.identifier.scopus2-s2.0-84872400694
dc.identifier.urihttp://hdl.handle.net/11449/73540
dc.language.isoeng
dc.relation.ispartofBrazilian Dental Journal
dc.relation.ispartofsjr0,476
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAcrylic resins
dc.subjectBiofilms
dc.subjectBrushing
dc.subjectCandida albicans
dc.subjectDenture cleansers
dc.subjectDisinfectants
dc.subjectOral hygiene
dc.titleEffectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viabilityen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/bdj/paboutj.htm
unesp.author.lattes8000248781842587[3]
unesp.author.lattes5737127334248549
unesp.author.lattes3003130522427820[6]
unesp.author.orcid0000-0002-7375-4714[6]
unesp.author.orcid0000-0002-2718-428X[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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