Apelin Enhances the Effects of Fusobacterium nucleatum on Periodontal Ligament Cells In Vitro

Página do item simplificado
dc.contributor.authorCores Ziskoven, Pablo
dc.contributor.authorNogueira, Andressa V. B.
dc.contributor.authorGutierrez, Lorena S. [UNESP]
dc.contributor.authorWeusmann, Jens
dc.contributor.authorEick, Sigrun
dc.contributor.authorBuduneli, Nurcan
dc.contributor.authorDeschner, James
dc.contributor.institutionUniversity Medical Center of the Johannes Gutenberg University
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of Bern
dc.contributor.institutionEge University
dc.date.accessioned2023-07-29T13:45:45Z
dc.date.available2023-07-29T13:45:45Z
dc.date.issued2023-03-01
dc.description.abstractThis study aimed to explore effects of Fusobacterium nucleatum with or without apelin on periodontal ligament (PDL) cells to better understand pathomechanistic links between periodontitis and obesity. First, the actions of F. nucleatum on COX2, CCL2, and MMP1 expressions were assessed. Subsequently, PDL cells were incubated with F. nucleatum in the presence and absence of apelin to study the modulatory effects of this adipokine on molecules related to inflammation and hard and soft tissue turnover. Regulation of apelin and its receptor (APJ) by F. nucleatum was also studied. F. nucleatum resulted in elevated COX2, CCL2, and MMP1 expressions in a dose- and time-dependent manner. Combination of F. nucleatum and apelin led to the highest (p < 0.05) expression levels of COX2, CCL2, CXCL8, TNF-α, and MMP1 at 48 h. The effects of F. nucleatum and/or apelin on CCL2 and MMP1 were MEK1/2- and partially NF-κB-dependent. The combined effects of F. nucleatum and apelin on CCL2 and MMP1 were also observed at protein level. Moreover, F. nucleatum downregulated (p < 0.05) the apelin and APJ expressions. In conclusion, obesity could contribute to periodontitis through apelin. The local production of apelin/APJ in PDL cells also suggests a role of these molecules in the pathogenesis of periodontitis.en
dc.description.affiliationDepartment of Periodontology and Operative Dentistry University Medical Center of the Johannes Gutenberg University
dc.description.affiliationDepartment of Diagnosis and Surgery School of Dentistry at Araraquara São Paulo State University-UNESP, SP
dc.description.affiliationLaboratory of Oral Microbiology Department of Periodontology University of Bern
dc.description.affiliationDepartment of Periodontology School of Dentistry Ege University
dc.description.affiliationUnespDepartment of Diagnosis and Surgery School of Dentistry at Araraquara São Paulo State University-UNESP, SP
dc.description.sponsorshipGerman-Israeli Foundation for Scientific Research and Development
dc.description.sponsorshipDeutscher Akademischer Austauschdienst
dc.description.sponsorshipIdGerman-Israeli Foundation for Scientific Research and Development: 1593/5-1
dc.description.sponsorshipIdDeutscher Akademischer Austauschdienst: 57589445
dc.identifierhttp://dx.doi.org/10.3390/ijms24054733
dc.identifier.citationInternational Journal of Molecular Sciences, v. 24, n. 5, 2023.
dc.identifier.doi10.3390/ijms24054733
dc.identifier.issn1422-0067
dc.identifier.issn1661-6596
dc.identifier.scopus2-s2.0-85149823532
dc.identifier.urihttp://hdl.handle.net/11449/248500
dc.language.isoeng
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.sourceScopus
dc.subjectapelin
dc.subjectAPJ
dc.subjectFusobacterium nucleatum
dc.subjectobesity
dc.subjectperiodontal ligament cells
dc.subjectperiodontitis
dc.titleApelin Enhances the Effects of Fusobacterium nucleatum on Periodontal Ligament Cells In Vitroen
dc.typeArtigo
unesp.author.orcid0000-0002-2756-5947[2]
unesp.author.orcid0000-0002-4323-5040[4]
unesp.author.orcid0000-0002-4619-2461[5]
unesp.author.orcid0000-0002-1590-5801[6]

Arquivos

Coleções

Artigos - Diagnóstico e Cirurgia - FOAR