MICROSATELLITE LOCI FOR ORTHOPHYTUM OPHIUROIDES (BROMELIOIDEAE, BROMELIACEAE) SPECIES ADAPTED TO NEOTROPICAL ROCK OUTCROPS

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dc.contributor.authorAoki-Goncalves, Felipe
dc.contributor.authorLouzada, Rafael B.
dc.contributor.authorDe Souza, Livia Moura
dc.contributor.authorPalma-Silva, Clarisse [UNESP]
dc.contributor.institutionInst Bot
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-03-18T15:56:21Z
dc.date.available2015-03-18T15:56:21Z
dc.date.issued2014-03-01
dc.description.abstractPremise of the study: Microsatellite primers were developed for Orthophytum ophiuroides, a rupicolous bromeliad species endemic to neotropical rocky fields. These microsatellite loci will be used to investigate population differentiation and species cohesion in such fragmented environments. The loci were tested for cross-amplification in related bromeliad species.Methods and Results: Eleven polymorphic microsatellite markers were isolated and characterized from an enriched library of O. ophiuroides. The loci were tested on 42 individuals from two populations of this species. The number of alleles per locus ranged from three to nine and the expected and observed heterozygosities ranged from 0.167 to 0.870 and from 0.369 to 0.958, respectively. Seven loci successfully amplified in other related bromeliad species.Conclusions: Our results suggest that the microsatellite loci developed here will be useful to assess genetic diversity and gene flow in O. ophiuroides for the investigation of population differentiation and species cohesion in neotropical mountainous habitats.en
dc.description.affiliationInst Bot, BR-04301902 Sao Paulo, Brazil
dc.description.affiliationUniv Fed Pernambuco, Dept Bot, BR-50670901 Recife, PE, Brazil
dc.description.affiliationUniv Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Ecol, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Ecol, BR-13506900 Rio Claro, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 11/06438-2
dc.description.sponsorshipIdFAPESP: 09/52725-3
dc.description.sponsorshipIdFAPESP: 09/17411-8
dc.format.extent4
dc.identifierhttp://dx.doi.org/10.3732/apps.1300073
dc.identifier.citationApplications In Plant Sciences. St Louis: Botanical Soc Amer Inc, v. 2, n. 3, 4 p., 2014.
dc.identifier.doi10.3732/apps.1300073
dc.identifier.fileWOS000342559000002.pdf
dc.identifier.issn2168-0450
dc.identifier.urihttp://hdl.handle.net/11449/117524
dc.identifier.wosWOS:000342559000002
dc.language.isoeng
dc.publisherBotanical Soc Amer Inc
dc.relation.ispartofApplications In Plant Sciences
dc.relation.ispartofjcr1.187
dc.relation.ispartofsjr0,746
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectBromeliaceaeen
dc.subjectcross-amplificationen
dc.subjectgene flowen
dc.subjectgenetic diversityen
dc.subjectOrthophytum ophiuroidesen
dc.subjectpopulation geneticsen
dc.titleMICROSATELLITE LOCI FOR ORTHOPHYTUM OPHIUROIDES (BROMELIOIDEAE, BROMELIACEAE) SPECIES ADAPTED TO NEOTROPICAL ROCK OUTCROPSen
dc.typeArtigo
dcterms.rightsHolderBotanical Soc Amer Inc
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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Artigos - Ecologia - IBRC