Genotoxicity in primary human peripheral lymphocytes after exposure to radiopacifiers in vitro

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dc.contributor.authorBraz, Mariana Gobbo [UNESP]
dc.contributor.authorMarcondes, Joao Paulo de Castro [UNESP]
dc.contributor.authorMatsumoto, Mariza Akemi
dc.contributor.authorDuarte, Marco Antonio Hungaro
dc.contributor.authorSalvadori, Daisy Maria Favero [UNESP]
dc.contributor.authorRibeiro, Daniel Araki
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:37:31Z
dc.date.available2014-05-20T13:37:31Z
dc.date.issued2008-02-01
dc.description.abstractTaking into consideration that DNA damage plays an important role in carcinogenesis, the purpose of this study was to evaluate whether some radiopacifiers widely used in clinical practice are able to induce genetic damage in primary human cells in vitro. Human peripheral lymphocytes obtained from 10 healthy volunteers were exposed to barium sulphate (BaSO(4)), zirconium oxide (ZnO(2)) and bismuth oxide (Bi(2)O(3)) at final concentrations ranging from 1 to 1000 mu g/mL for 1 h at 37 degrees C. The negative control group was treated with vehicle control (phosphate buffer solution) for 1 h at 37 degrees C and the positive control group was treated with hydrogen peroxide (at 100 mu M) for 5 min on ice. Results were analyzed by the Friedman non-parametric test. The results pointed all compounds tested out did not induce DNA breakage in human peripheral lymphocytes as depicted by the mean tail moment and tail intensity in all concentrations tested. In summary, our results indicate that exposure to these radiopacifiers may not be a factor that increases the level of DNA lesions in human peripheral lymphocytes as detected by single cell gel (comet) assay.en
dc.description.affiliationUniv Fed São Paulo, Dept Hlth Sci, BR-11060001 Santos, SP, Brazil
dc.description.affiliationUniv Sagrado Coracao, Dept Dent Clin, Bauru, SP, Brazil
dc.description.affiliationUniv São Paulo, Botucatu Med Sch, Dept Pathol, UNESP, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv São Paulo, Botucatu Med Sch, Dept Pathol, UNESP, Botucatu, SP, Brazil
dc.format.extent601-605
dc.identifierhttp://dx.doi.org/10.1007/s10856-007-3000-2
dc.identifier.citationJournal of Materials Science-materials In Medicine. Dordrecht: Springer, v. 19, n. 2, p. 601-605, 2008.
dc.identifier.doi10.1007/s10856-007-3000-2
dc.identifier.issn0957-4530
dc.identifier.lattes5051118752980903
dc.identifier.lattes5530023010203804
dc.identifier.lattes8621510886887389
dc.identifier.orcid0000-0001-5389-0105
dc.identifier.orcid0000-0003-4413-226X
dc.identifier.urihttp://hdl.handle.net/11449/12991
dc.identifier.wosWOS:000252976100016
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofJournal of Materials Science: Materials in Medicine
dc.relation.ispartofjcr2.448
dc.relation.ispartofsjr0,647
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleGenotoxicity in primary human peripheral lymphocytes after exposure to radiopacifiers in vitroen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes5051118752980903
unesp.author.lattes5530023010203804
unesp.author.lattes8621510886887389
unesp.author.orcid0000-0001-5389-0105[3]
unesp.author.orcid0000-0001-9323-3134[5]
unesp.author.orcid0000-0003-3051-737X[4]
unesp.author.orcid0000-0002-5116-2494[2]
unesp.author.orcid0000-0003-4413-226X[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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