Effect of Curcumin-Nanoemulsion Associated with Photodynamic Therapy in Cervical Carcinoma Cell Lines

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dc.contributor.authorDe Matos, Renata Prandini Adum [UNESP]
dc.contributor.authorCalmon, Marilia Freitas [UNESP]
dc.contributor.authorAmantino, Camila Fernanda
dc.contributor.authorVilla, Luisa Lina
dc.contributor.authorPrimo, Fernando Lucas [UNESP]
dc.contributor.authorTedesco, Antonio Claudio
dc.contributor.authorRahal, Paula [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionInstituto de Câncer do Estado de São Paulo (ICESP)
dc.date.accessioned2018-12-11T17:19:38Z
dc.date.available2018-12-11T17:19:38Z
dc.date.issued2018-01-01
dc.description.abstractCervical cancer is the fourth cause of cancer death in women. Curcumin has antineoplastic properties. Furthermore, curcumin may be used as a photosensitizing agent in Photodynamic Therapy. This study aimed to investigate the effects of Photodynamic Therapy in cellular viability using curcumin-nanoemulsion as a photosensitizing drug in cervical carcinoma cell lines. The empty nanoemulsion presented very low cytotoxicity in all cell lines analyzed. Additionally, the incubation with curcumin-nanoemulsion at 20 μM of curcumin showed more than 80% of cell viability for cell lines. Nanoemulsions were shown to be internalized inside cells by fluorescence microscopy and were observed in the intracellular environment for up to 36 hours after incubation with cell lines. In addition, after the Photodynamic Therapy we observed a high phototoxic effect of the curcumin-nanoemulsion with less than 5% of viable cells after irradiation. This was accompanied by an increase in caspase-3/caspase-7 activities after cell treatment with curcumin-nanoemulsion and Photodynamic Therapy, suggesting cell death by apoptosis. We conclude that the curcumin-nanoemulsion formulation behaves as a photosensitizing drug in Photodynamic Therapy and shows potential as an alternative treatment to cervical lesions using an endoscopic diode fiber laser setup for in situ activation or cavity activation using a diffuse fiber delivery system.en
dc.description.affiliationInstitute of Biosciences Humanities and Exact Sciences (IBILCE) Department of Biology São Paulo State University (UNESP), Rua Cristovão Colombo, 2265 Bairro Jardim Nazareth
dc.description.affiliationDepartment of Chemistry Center of Nanotechnology and Tissue Engineering Photobiology and Photomedicine Research Group Faculty of Philosophy Sciences and Letters of Ribeirão Preto University of São Paulo, Av. Bandeirantes 3900
dc.description.affiliationCenter for Translational Research in Oncology Instituto de Câncer do Estado de São Paulo (ICESP), Av. Dr. Arnaldo 251, Bairro Cerqueira César
dc.description.affiliationFaculty of Pharmaceutical Sciences of Araraquara Department of Bioprocess and Biotechnology São Paulo State University (UNESP), Rodovia Araraquara/Jaú, Km 01, s/n, Campos Ville
dc.description.affiliationFaculdade de Medicina Department of Radiology and Oncology Universidade de São Paulo (USP), Av. Dr. Arnaldo 251, Bairro Cerqueira César
dc.description.affiliationUnespInstitute of Biosciences Humanities and Exact Sciences (IBILCE) Department of Biology São Paulo State University (UNESP), Rua Cristovão Colombo, 2265 Bairro Jardim Nazareth
dc.description.affiliationUnespFaculty of Pharmaceutical Sciences of Araraquara Department of Bioprocess and Biotechnology São Paulo State University (UNESP), Rodovia Araraquara/Jaú, Km 01, s/n, Campos Ville
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2013/04286-6
dc.identifierhttp://dx.doi.org/10.1155/2018/4057959
dc.identifier.citationBioMed Research International, v. 2018.
dc.identifier.doi10.1155/2018/4057959
dc.identifier.file2-s2.0-85045881478.pdf
dc.identifier.issn2314-6141
dc.identifier.issn2314-6133
dc.identifier.lattes7991082362671212
dc.identifier.orcid0000-0001-5693-6148
dc.identifier.scopus2-s2.0-85045881478
dc.identifier.urihttp://hdl.handle.net/11449/176216
dc.language.isoeng
dc.relation.ispartofBioMed Research International
dc.relation.ispartofsjr0,935
dc.relation.ispartofsjr0,935
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.titleEffect of Curcumin-Nanoemulsion Associated with Photodynamic Therapy in Cervical Carcinoma Cell Linesen
dc.typeArtigo
unesp.author.lattes7991082362671212[7]
unesp.author.orcid0000-0001-5203-0103[2]
unesp.author.orcid0000-0001-5693-6148[7]

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