Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis

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dc.contributor.authordos Santos Alves, Taila
dc.contributor.authorRosa, Vinícius Sanches
dc.contributor.authorda Silva Leite, Domingos
dc.contributor.authorGuerra, Simony Trevizan [UNESP]
dc.contributor.authorJoaquim, Sâmea Fernandes [UNESP]
dc.contributor.authorGuimarães, Felipe Freitas [UNESP]
dc.contributor.authorde Figueiredo Pantoja, José Carlos [UNESP]
dc.contributor.authorLucheis, Simoni Baldini [UNESP]
dc.contributor.authorRall, Vera Lúcia Mores [UNESP]
dc.contributor.authorHernandes, Rodrigo Tavanelli [UNESP]
dc.contributor.authorLangoni, Helio [UNESP]
dc.contributor.authorRibeiro, Márcio Garcia [UNESP]
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T13:39:41Z
dc.date.available2023-07-29T13:39:41Z
dc.date.issued2023-03-01
dc.description.abstractMastitis occurrence in dairy cows is a broad topic that involves several sectors, from antimicrobial resistance and virulence of strains to economic implications and cattle management practices. Here, we assessed the molecular characterization (antimicrobial resistance determinants, virulence genes, sequences type, serotypes, and plasmid types) of 178 Escherichia coli strains isolated from milk samples from cows with clinical mastitis using a genome-based k-mers approach. Of these, 53 (29.8%) showed multidrug resistance by disc diffusion. We selected eight multidrug-resistant mastitis-associated E. coli for whole-genome sequencing and molecular characterization based on raw data using k-mers. We assessed antimicrobial resistance genes, virulence factors, serotypes, Multilocus Sequence Typing (MLST), and plasmid types. The most antimicrobial resistance gene found were blaTEM-1B (7/8), tetA (6/8), strA (6/8), strB (6/8), and qnrB19 (5/8). A total of 25 virulence factors were detected encoding adhesins, capsule, enzymes/proteins, increased serum survival, hemolysin, colicins, and iron uptake. These virulence factors were associated with Extraintestinal Pathogenic E. coli. Three pandemic clones were found: ST10, ST101, and ST69. Two E. coli were assigned in the O117 serogroup and one in the O8:H25 serotype. The most common plasmid groups were IncFII (7/8) and IncFIB (6/8). Our findings contribute to the knowledge of virulence mechanisms, epidemiological aspects, and antimicrobial resistance determinants of E. coli strains obtained from clinical mammary infections of cows.en
dc.description.affiliationDepartment of Genetics Evolution Microbiology and Immunology Institute of Biology University of Campinas-UNICAMP, SP
dc.description.affiliationDepartment of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Sciences São Paulo State University-UNESP, SP
dc.description.affiliationDepartment of Microbiology and Immunology São Paulo State University-UNESP, SP
dc.description.affiliationUnespDepartment of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Sciences São Paulo State University-UNESP, SP
dc.description.affiliationUnespDepartment of Microbiology and Immunology São Paulo State University-UNESP, SP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2015/19688-8
dc.identifierhttp://dx.doi.org/10.1007/s00284-023-03191-6
dc.identifier.citationCurrent Microbiology, v. 80, n. 3, 2023.
dc.identifier.doi10.1007/s00284-023-03191-6
dc.identifier.issn1432-0991
dc.identifier.issn0343-8651
dc.identifier.scopus2-s2.0-85147178135
dc.identifier.urihttp://hdl.handle.net/11449/248288
dc.language.isoeng
dc.relation.ispartofCurrent Microbiology
dc.sourceScopus
dc.titleGenome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitisen
dc.typeArtigo
unesp.author.orcid0000-0002-5672-509X[1]

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Artigos - Produção Animal - FMVZ
Artigos - Microbiologia e Imunologia - IBB