Citoinclusão de capa leucocitária e medula óssea de cães: padronização da técnica

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dc.contributor.authorPaiva, M. B. [UNESP]
dc.contributor.authorGouveia, G. M. [UNESP]
dc.contributor.authorFioravanti, H. [UNESP]
dc.contributor.authorLuvizotto, Maria Cecília Rui [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-12-03T13:08:41Z
dc.date.available2014-12-03T13:08:41Z
dc.date.issued2014-04-01
dc.description.abstractThe cell block technique is widely used and recognized by health professionals, but lacks in information regarding the specific contribution of this method to veterinary medicine. The cytology of bone marrow and buffy coat (cellular portion of the hole blood) are widely used in the diagnosis of neoplastic and nonneoplastic diseases, and also for the detection of infectious agents. Although the importance of these samples is widely recognized, there is no information about the use of buffy coat and bone marrow samples in the cell block procedure among the research material used for this paper. This work aims for the cell block standardization for canine buffy coat and bone marrow samples. We collected 110 buffy coat samples and 44 bone marrow samples for the cell block preparation, and 68.2% of buffy coat and bone marrow proved to be viable at the end of the procedure. The 95% ethanol fixatives along with the 20 minute processing steps (absolute ethanol, xilol and paraffin, 3 of each) were crucial for the quality of the material both in microtomy and optical microscopy. Mechanical separation of the buffy coat proved to be easy and cheap and was used to compose the cell block technique. In this research we emphasized the importance of cell block standardization in order to develop and easy, inexpensive and reproducible method, regardless of any of the professionals' limitations.en
dc.description.affiliationUNESP, Fac Med Vet, Aracatuba, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Med Vet, Aracatuba, SP, Brazil
dc.format.extent439-444
dc.identifierhttp://dx.doi.org/10.1590/1678-41626125
dc.identifier.citationArquivo Brasileiro De Medicina Veterinaria E Zootecnia. Minas Gerais: Arquivo Brasileiro Medicina Veterinaria Zootecnia, v. 66, n. 2, p. 439-444, 2014.
dc.identifier.doi10.1590/1678-41626125
dc.identifier.fileS0102-09352014000200017.pdf
dc.identifier.issn0102-0935
dc.identifier.lattes4584674909952477
dc.identifier.scieloS0102-09352014000200017
dc.identifier.urihttp://hdl.handle.net/11449/111474
dc.identifier.wosWOS:000337146700017
dc.language.isopor
dc.publisherArquivo Brasileiro Medicina Veterinaria Zootecnia
dc.relation.ispartofArquivo Brasileiro de Medicina Veterinária e Zootecnia
dc.relation.ispartofjcr0.286
dc.relation.ispartofsjr0,248
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectdogen
dc.subjectcell blocken
dc.subjectbuffy coaten
dc.subjectbone marrowen
dc.subjecttechniqueen
dc.titleCitoinclusão de capa leucocitária e medula óssea de cães: padronização da técnicapt
dc.title.alternativeCell block of canine buffy coat and bone marrow in paraffin blocks: technique standardizationen
dc.typeArtigo
dcterms.rightsHolderArquivo Brasileiro Medicina Veterinaria Zootecnia
unesp.author.lattes4584674909952477
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt

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Artigos - Clínica, Cirurgia e Reprodução Animal - FMVA