Efficiency of cryoprotectors for cryopreservation of two orchid species from Americas

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dc.contributor.authorPereira, Suzana Targanski Sajovic [UNESP]
dc.contributor.authorVendrame, Wagner Aparecido
dc.contributor.authorPivetta, Kathia Fernandes Lopes [UNESP]
dc.contributor.authorSorgato, José Carlos
dc.contributor.authorde Faria, Ricardo Tadeu
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionInstitute of Food and Agricultural Sciences
dc.contributor.institutionFaculdade de Ciências Agrárias
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.date.accessioned2022-05-01T13:11:35Z
dc.date.available2022-05-01T13:11:35Z
dc.date.issued2021-01-01
dc.description.abstractThe objective of this study was to evaluate the efficiency of cryoprotective solution (PVS2) combined with phloroglucinol for the cryopreservation of seeds of two orchid species, Encyclia cordigera and Epidendrum ciliare. Seeds of Encyclia cordigera had 91.03% initial viability and 91.99% germination. The treatment of the seeds with PVS2 at 0°C with 1% phloroglucinol for 60 min returned 93.79% viability and 91.01% germination after recovery from LN, consequently resulting in faster development of protocorms. For Epidendrum ciliare, seed viability was 85.65% and germination was 85.90%. Seed exposure to the PVS2 at 0°C with 1% phloroglucinol for 180 min showed viability of 39.23% and germination of 37.88%. Despite lower germination, 78.90% of the protocorms reached stage P3 of development, when evaluated 45 days after sowing, not significantly different from the control 1, and showed normal development. These results indicate that PVS2 cryoprotective solution is efficient when combined with phloroglucinol for the cryopreservation and successful recovery of seeds of Encyclia cordigera and Epidendrum ciliare. The present study also indicates that response to cryopreservation and success of recovery after cold storage is species-specific and requires adjustments in exposure time to PVS2 at 0°C prior to immersion in LN.en
dc.description.affiliationSão Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, Via de Acesso Prof. Paulo Donato Castellane s/n, SP
dc.description.affiliationniversity of Florida Environmental Horticulture Department Tropical Research and Education Center Institute of Food and Agricultural Sciences, 18905 SW 280th St
dc.description.affiliationUniversidade Federal da Grande Dourados Faculdade de Ciências Agrárias, Rod. Dourados/Itahum km 12 C.P. 364, MS
dc.description.affiliationUniversidade Estadual de Londrina Centro de Ciências Agrarias Depto. Agronomia, Rod. Celso Garcia Cid (PR 445) km 380 C.P. 10.011, PR
dc.description.affiliationUnespSão Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, Via de Acesso Prof. Paulo Donato Castellane s/n, SP
dc.identifierhttp://dx.doi.org/10.1590/2175-7860202172123
dc.identifier.citationRodriguesia, v. 72.
dc.identifier.doi10.1590/2175-7860202172123
dc.identifier.issn2175-7860
dc.identifier.issn0370-6583
dc.identifier.scopus2-s2.0-85123772157
dc.identifier.urihttp://hdl.handle.net/11449/234070
dc.language.isoeng
dc.relation.ispartofRodriguesia
dc.sourceScopus
dc.subjectEncyclia cordigera
dc.subjectEpidendrum ciliare
dc.subjectGermplasm conservation
dc.subjectOrchidaceae
dc.subjectPVS2
dc.titleEfficiency of cryoprotectors for cryopreservation of two orchid species from Americasen
dc.typeArtigo
unesp.author.orcid0000-0003-0424-489X[1]
unesp.author.orcid0000-0001-6391-7623[2]
unesp.author.orcid0000-0001-9983-2402[3]
unesp.author.orcid0000-0003-0001-5467[4]
unesp.author.orcid0000-0002-7595-1965[5]

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