Effects of fibrin sealant and bone fragments on defect regeneration performed on rat tibiae: An experimental study

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dc.contributor.authorBarbosa de Oliveira, Carla Teresa
dc.contributor.authorLeonel, Bruna Carlos
dc.contributor.authorOliveira, Ana Clara de
dc.contributor.authorPaiva, Marcela de Brito
dc.contributor.authorRamos, Junia
dc.contributor.authorBarraviera, Benedito [UNESP]
dc.contributor.authorFerreira Junior, Rui Seabra [UNESP]
dc.contributor.authorShimano, Antonio Carlos
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-10T19:55:40Z
dc.date.available2020-12-10T19:55:40Z
dc.date.issued2020-04-01
dc.description.abstractFibrin sealant (FS) is a biomaterial that exhibits hemostatic and repairing properties. It has been successfully used as scaffolds and adhesives to improve repair and regeneration of tissues. The objective of this study was to evaluate the effect of FS in the regeneration process of bone defects in male rat tibias through macroscopic, microscopic and mechanical analysis. A bone defect of 2.9 mm was performed on the medial face of the proximal third of the tibia of 40 rats and implanted FS and autologous bone graft (AG). The animals were divided into four groups: animals with bone defect without any treatment (CON), animals treated with fibrin sealant (TFS), animals treated with autologous graft (TAG) and animals treated with fibrin sealant and autologous graft (FSAG). The animals were euthanized 42 days after surgery. Macroscopic analysis showed no difference between the groups (p > 0.05) in relation to tibial weight, but a statistically significant difference (p = 0.005) was observed for their length. Micro-computed tomography (micro-CT) revealed tendentious values regarding bone microarchitecture and FS. Bone mineral densitometry (BMD) showed significance between the FSAG (p = 0.009) and TFS (p = 0.007) groups. The bone mineral content (BMC) presented a significant difference between all groups (p = 0.020). Maximum strength showed a significant difference between the FSAG group (p = 0.007) and the others. The results obtained in relation to the relative stiffness also present a significant difference (p = 0.023). Newly formed bone showed significant differences between groups (p = 0.035). We conclude that bone defect regeneration was directly influenced by the use of FS and AG.en
dc.description.affiliationUniv Sao Paulo, Med Sch Ribeirao Preto, Bioengn Lab, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Dent Sch Ribeirao Preto, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP, Brazil
dc.description.affiliationSao Paulo State Univ, Ctr Study Venoms & Venomous Anim, Rua Jose Barbosa de Barros, BR-18610307 Botucatu, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Ctr Study Venoms & Venomous Anim, Rua Jose Barbosa de Barros, BR-18610307 Botucatu, SP, Brazil
dc.format.extent8
dc.identifierhttp://dx.doi.org/10.1016/j.jmbbm.2020.103662
dc.identifier.citationJournal Of The Mechanical Behavior Of Biomedical Materials. Amsterdam: Elsevier, v. 104, 8 p., 2020.
dc.identifier.doi10.1016/j.jmbbm.2020.103662
dc.identifier.issn1751-6161
dc.identifier.urihttp://hdl.handle.net/11449/196770
dc.identifier.wosWOS:000525737000017
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal Of The Mechanical Behavior Of Biomedical Materials
dc.sourceWeb of Science
dc.subjectFibrin tissue adhesive
dc.subjectBone regeneration
dc.subjectAutologous transplantation
dc.subjectTibia
dc.subjectRats
dc.titleEffects of fibrin sealant and bone fragments on defect regeneration performed on rat tibiae: An experimental studyen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes6840524602748457[6]
unesp.author.orcid0000-0002-9855-5594[6]

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Botucatu - CEVAP - Centro de Estudos de Venenos e Animais Peçonhentos