Sevoflurane induces DNA damage whereas isoflurane leads to higher antioxidative status in anesthetized rats

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dc.contributor.authorRocha, Thalita Leone Alves [UNESP]
dc.contributor.authorDias-Junior, Carlos Alan [UNESP]
dc.contributor.authorPossomato-Vieira, José Sergio [UNESP]
dc.contributor.authorGonçalves-Rizzi, Victor Hugo [UNESP]
dc.contributor.authorNogueira, Flávia Ribeiro [UNESP]
dc.contributor.authorSouza, Kátina Meneghetti de [UNESP]
dc.contributor.authorBraz, Leandro Gobbo [UNESP]
dc.contributor.authorBraz, Mariana Gobbo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-10-21T13:11:06Z
dc.date.available2015-10-21T13:11:06Z
dc.date.issued2015-01-01
dc.description.abstractTaking into account that there are controversial antioxidative effects of inhalational anesthetics isoflurane and sevoflurane and absence of comparison of genotoxicity of both anesthetics in animal model, the aim of this study was to compare DNA damage and antioxidant status in Wistar rats exposed to a single time to isoflurane or sevoflurane. The alkaline single-cell gel electrophoresis assay (comet assay) was performed in order to evaluate DNA damage in whole blood cells of control animals (unexposed; n = 6) and those exposed to 2% isoflurane (n = 6) or 4% sevoflurane (n = 6) for 120 min. Plasma antioxidant status was determined by 3( 4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. There was no statistically significant difference between isoflurane and sevoflurane groups regarding hemodynamic and temperature variables (P > 0.05). Sevoflurane significantly increased DNA damage compared to unexposed animals (P = 0.02). In addition, Wistar rats anesthetized with isoflurane showed higher antioxidative status (MTT) than control group (P = 0.019). There were no significant differences in DNA damage or antioxidant status between isoflurane and sevoflurane groups (P > 0.05). In conclusion, our findings suggest that, in contrast to sevoflurane exposure, isoflurane increases systemic antioxidative status, protecting cells from DNA damage in rats.en
dc.description.affiliationUnespUniversidade Estadual Paulista, Departamento de Farmacologia, Instituto de Biociências de Botucatu
dc.description.affiliationUnespUniversidade Estadual Paulista, Departamento de Anestesiologia, Faculdade de Medicina de Botucatu
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent1-6
dc.identifierhttp://www.hindawi.com/journals/bmri/2015/264971/
dc.identifier.citationBiomed Research International. New York: Hindawi Publishing Corporation, v. 2015, p. 1-6, 2015.
dc.identifier.doi10.1155/2015/264971
dc.identifier.fileWOS000355805900001.pdf
dc.identifier.issn2314-6133
dc.identifier.lattes7199562550978496
dc.identifier.lattes5530023010203804
dc.identifier.orcid0000-0003-4413-226X
dc.identifier.urihttp://hdl.handle.net/11449/128572
dc.identifier.wosWOS:000355805900001
dc.language.isoeng
dc.publisherHindawi Publishing Corporation
dc.relation.ispartofBiomed Research International
dc.relation.ispartofjcr2.583
dc.relation.ispartofsjr0,935
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleSevoflurane induces DNA damage whereas isoflurane leads to higher antioxidative status in anesthetized ratsen
dc.typeArtigo
dcterms.rightsHolderHindawi Publishing Corporation
unesp.author.lattes5530023010203804
unesp.author.lattes6296664642422599[2]
unesp.author.orcid0000-0002-1927-8729[7]
unesp.author.orcid0000-0003-4413-226X[8]
unesp.author.orcid0000-0002-0348-6144[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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Artigos - Farmacologia - IBB
Artigos - Anestesiologia - FMB