Rosmarinic acid, a new snake venom phospholipase A(2) inhibitor from Cordia verbenacea (Boraginaceae): antiserum action potentiation and molecular interaction

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dc.contributor.authorTicli, F. K.
dc.contributor.authorHage, LIS
dc.contributor.authorCambraia, R. S.
dc.contributor.authorPereira, P. S.
dc.contributor.authorMagro, A. J.
dc.contributor.authorFontes, MRM
dc.contributor.authorStabeli, R. G.
dc.contributor.authorGiglio, JR
dc.contributor.authorFranca, S. C.
dc.contributor.authorSoares, A. M.
dc.contributor.authorSampaio, S. V.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUNAERP
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUNIR
dc.date.accessioned2014-05-20T13:49:21Z
dc.date.available2014-05-20T13:49:21Z
dc.date.issued2005-09-01
dc.description.abstractMany plants are used in traditional medicine as active agents against various effects induced by snakebite. The methanolic extract from Cordia verbenacea (Cv) significantly inhibited paw edema induced by Bothrops jararacussu snake venom and by its main basic phospholipase A(2) homologs, namely bothropstoxins I and II (BthTXs). The active component was isolated by chromatography on Sephadex LH-20 and by RP-HPLC on a C18 column and identified as rosmarinic acid (Cv-RA). Rosmarinic acid is an ester of caffeic acid and 3,4-dihydroxyphenyllactic acid [2-O-cafeoil-3-(3,4-di-hydroxy-phenyl)-R-lactic acid]. This is the first report of RA in the species C. verbenacea ('baleeira', 'whaler') and of its anti-inflammatory and antimyotoxic properties against snake venoms and isolated toxins. RA inhibited the edema and myotoxic activity induced by the basic PLA(2)s BthTX-I and BthTX-II. It was, however, less efficient to inhibit the PLA(2) activity of BthTX-II and, still less, the PLA(2) and edema-inducing activities of the acidic isoform BthA-1-PLA(2), from the same venom, showing therefore a higher inhibitory activity upon basic PLA(2)s. RA also inhibited most of the myotoxic and partially the edema-inducing effects of both basic PLA(2)s, thus reinforcing the idea of dissociation between the catalytic and pharmacological domains. The pure compound potentiated the ability of the commercial equine polyvalent antivenom in neutralizing lethal and myotoxic effects of the crude venom and of isolated PLA(2)s in experimental models. CD data presented here suggest that, after binding, no significant conformation changes occur either in the Cv-RA or in the target PLA(2). A possible model for the interaction of rosmarinic acid with Lys49-PLA(2) BthTX-I is proposed. (c) 2005 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniv São Paulo, FCFRP, Dept Analises Clin Toxicol & Bromatol, Ribeirao Preto, Brazil
dc.description.affiliationUNAERP, Unidade Biotecnol, Ribeirao Preto, Brazil
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, Dept Fis & Biofis, Botucatu, SP, Brazil
dc.description.affiliationUNIR, FioCruz, Lab Bioquim, Inst Pesquisas Patol Trop, Porto Velho, RO, Brazil
dc.description.affiliationUniv São Paulo, FMRP, Dept Bioquim & Imunol, Ribeirao Preto, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, Dept Fis & Biofis, Botucatu, SP, Brazil
dc.format.extent318-327
dc.identifierhttp://dx.doi.org/10.1016/j.toxicon.2005.04.023
dc.identifier.citationToxicon. Oxford: Pergamon-Elsevier B.V., v. 46, n. 3, p. 318-327, 2005.
dc.identifier.doi10.1016/j.toxicon.2005.04.023
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/11449/17580
dc.identifier.wosWOS:000231405900010
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofToxicon
dc.relation.ispartofjcr2.352
dc.relation.ispartofsjr0,692
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCordia verbenaceapt
dc.subjectrosmarinic acidpt
dc.subjectanti-inflammatorypt
dc.subjectantimyotoxicpt
dc.subjectantiophidianpt
dc.subjectphospholipase A(2) inhibitorpt
dc.subjectBothrops jararacussupt
dc.subjectsnake venompt
dc.titleRosmarinic acid, a new snake venom phospholipase A(2) inhibitor from Cordia verbenacea (Boraginaceae): antiserum action potentiation and molecular interactionen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes0059017255172730[5]
unesp.author.orcid0000-0002-4634-6221[6]
unesp.author.orcid0000-0003-4864-430X[9]
unesp.author.orcid0000-0002-0476-920X[7]
unesp.author.orcid0000-0003-3274-180X[11]
unesp.author.orcid0000-0002-0155-8968[4]
unesp.author.orcid0000-0002-4253-6992[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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