Near-infrared Raman spectroscopy to detect anti-Toxoplasma gondii antibodies in blood sera of domestic cats

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dc.contributor.authorDuarte, J.
dc.contributor.authorTadeu, M.
dc.contributor.authorPacheco, T.
dc.contributor.authorSilveira, L.
dc.contributor.authorMachado, R. Z. [UNESP]
dc.contributor.authorMartins, R. A.
dc.contributor.authorZangaro, R. A.
dc.contributor.authorVillaverde, A. Balbin
dc.contributor.institutionUniversidade Do Vale Do Paraiba
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-28T19:55:18Z
dc.date.available2022-04-28T19:55:18Z
dc.date.issued2001-01-01
dc.description.abstractNear Infrared (NIR) Raman Spectroscopy has been study for the last years for many biomedical applications. It is a powerful tool for biological materials analysis. Toxoplasmosis is an important zoonosis in public health, being the cats the principal responsible for the transmission of the disease in Brazil. The objective of this work is to investigate a new method of diagnosis of this disease. NIR Raman Spectroscopy was used to detect anti Toxoplasma gondii antibodies in blood sera from domestic cats, without sample preparation. In all, six blood serum samples were used for this study. A previous serological test was done by the Indirect Immunoenzymatic Assay (ELISA) to permit a comparative study between both techniques and it showed that three serum samples were positive and the other three were negative to toxoplasmosis. Raman spectra were taken for all the samples and analyzed by using the Principal Components Analysis (PCA). A diagnosis parameter was defined from the analysis of the second and third principal components of the Raman spectra. It was found that this parameter can detect the infection level of the animal. The results have indicated that NIR Raman Spectroscopy, associated to the PCA can be a promising technique for serological analysis, such as toxoplasmosis, allowing a fast and sensitive method of diagnosis.en
dc.description.affiliationInst. De Pesquisa E Desenvolvimento Universidade Do Vale Do Paraiba, Av. Shishima hifumi 2911, 12244-000 São josé dos campos, SP
dc.description.affiliationDepto. Patologia Veterinária - Universidade Estadual Paulista, Rodovia Carlos Tonnani-km 5, 14870-000, Jaboticabal, SP
dc.description.affiliationUnespDepto. Patologia Veterinária - Universidade Estadual Paulista, Rodovia Carlos Tonnani-km 5, 14870-000, Jaboticabal, SP
dc.format.extent536-543
dc.identifierhttp://dx.doi.org/10.1117/12.427767
dc.identifier.citationProceedings of SPIE - The International Society for Optical Engineering, v. 4244, p. 536-543.
dc.identifier.doi10.1117/12.427767
dc.identifier.issn0277-786X
dc.identifier.scopus2-s2.0-0034943955
dc.identifier.urihttp://hdl.handle.net/11449/224206
dc.language.isoeng
dc.relation.ispartofProceedings of SPIE - The International Society for Optical Engineering
dc.sourceScopus
dc.subjectDisease diagnosis
dc.subjectNIR Raman spectroscopy
dc.subjectPCA method
dc.subjectSerological analysis
dc.subjectToxoplasmosis
dc.titleNear-infrared Raman spectroscopy to detect anti-Toxoplasma gondii antibodies in blood sera of domestic catsen
dc.typeArtigo

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