Comparison of two different methods for detecting periodontal pathogenic bacteria

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dc.contributor.authorBedran, Telma Blanca Lombardo
dc.contributor.authorde Oliveira, Guilherme José Pimentel Lopes
dc.contributor.authorSpolidorio, Luís Carlos
dc.contributor.authorCirelli, Joni Augusto
dc.contributor.authorSpolidorio, Denise Palomari
dc.contributor.institutionNove de Julho University
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2022-04-29T08:45:19Z
dc.date.available2022-04-29T08:45:19Z
dc.date.issued2016-01-01
dc.description.abstractAim: To perform a comparative analysis between two methods for detecting Porphyromonas gingivalis, Tannerella forsythia and Porphyromonas endodontalis in periodontal plaque samples.Methods: The study sample consisted of twenty systemically healthy patients showing generalized chronic periodontitis. The subgingival samples for microbiological analysis were collected before (baseline) and 60 days after a basic periodontal therapy from 30 non-adjacent affected sites (Probing Depth (PD): 5-7 mm, Clinical Attachment Loss (CAL) ≥ 5 mm, positive for Bleeding on Probing (BOP)). Microbiological analysis was performed by PCR and qPCR. To allow a comparative analysis between both methods, qPCR was divided in three different scores (score 2: presence of more than 100 bacteria; score 1: presence of 10-100 bacteria, and score 0: absence of bacteria), in accordance to DNA quantity, while for PCR two scores were assigned: presence or absence of bacteria. Results: qPCR demonstrated higher sensitivity in the detection of these pathogens compared with PCR when scores 1 and 2 were considered positive. However, when only score 2 was considered positive, PCR and qPCR showed better agreement. Conclusions: qPCR demonstrated higher sensitivity than conventional PCR for detection of low numbers of microorganisms and can be useful for the quantification of periodontopathogens.en
dc.description.affiliationDepartment of Dentistry Nove de Julho University
dc.description.affiliationDepartment of Oral Diagnosis and Surgery Araraquara Dental School State University of São Paulo
dc.description.affiliationDepartment of Physiology and Pathology Araraquara Dental School State University of São Paulo
dc.format.extent166-172
dc.identifierhttp://dx.doi.org/10.20396/bjos.v15i3.8649599
dc.identifier.citationBrazilian Journal of Oral Sciences, v. 15, n. 3, p. 166-172, 2016.
dc.identifier.doi10.20396/bjos.v15i3.8649599
dc.identifier.issn1677-3225
dc.identifier.issn1677-3217
dc.identifier.scopus2-s2.0-85028870262
dc.identifier.urihttp://hdl.handle.net/11449/231408
dc.language.isoeng
dc.relation.ispartofBrazilian Journal of Oral Sciences
dc.sourceScopus
dc.subjectBacteria
dc.subjectPeriodontal diseases
dc.subjectPolymerase chain reaction
dc.titleComparison of two different methods for detecting periodontal pathogenic bacteriaen
dc.typeArtigo

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