Heterologous fibrin sealant potentiates axonal regeneration after peripheral nerve injury with reduction in the number of suture points

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dc.contributor.authorLeite, Ana Paula Silveira [UNESP]
dc.contributor.authorPinto, Carina Guidi [UNESP]
dc.contributor.authorTibúrcio, Felipe Cantore [UNESP]
dc.contributor.authorSartori, Arthur Alves [UNESP]
dc.contributor.authorde Castro Rodrigues, Antonio
dc.contributor.authorBarraviera, Benedito [UNESP]
dc.contributor.authorFerreira, Rui Seabra [UNESP]
dc.contributor.authorFiladelpho, André Luis [UNESP]
dc.contributor.authorMatheus, Selma Maria Michelin [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUnifai-Centro Universitário de Adamantina
dc.date.accessioned2019-10-06T16:21:33Z
dc.date.available2019-10-06T16:21:33Z
dc.date.issued2019-04-01
dc.description.abstractThe use of suture associated with heterologous fibrin sealant has been highlighted for reconstruction after peripheral nerve injury, having the advantage of being safe for clinical use. In this study we compared the use of this sealant associated with reduced number of stitches with conventional suture after ischiatic nerve injury. 36 Wistar rats were divided into 4 groups: Control (C), Denervated (D), ischiatic nerve neurotmesis (6 mm gap); Suture (S), epineural anastomosis after 7 days from neurotmesis, Suture + Fibrin Sealant (SFS), anastomosis with only one suture point associated with Fibrin Sealant. Catwalk, electromyography, ischiatic and tibial nerve, soleus muscle morphological and morphometric analyses were performed. The amplitude and latency values of the Suture and Suture + Fibrin Sealant groups were similar and indicative of nerve regeneration.The ischiatic nerve morphometric analysis in the Suture + Fibrin Sealant showed superior values related to axons and nerve fibers area and diameter when compared to Suture group. In the Suture and Suture + Fibrin Sealant groups, there was an increase in muscle weight and in fast fibers frequency, it was a decrease in the percentage of collagen compared to group Denervated and in the neuromuscular junctions, the synaptic boutons were reestablished.The results suggest a protective effect at the lesion site caused by the fibrin sealant use. The stitches reduction minimizes the trauma caused by the needle and it accelerates the surgical practice. So the heterologous fibrin sealant use in nerve reconstruction should be considered.en
dc.description.affiliationGraduate Program on the General Bases of Surgery Botucatu Medical School Department of Anatomy Universidade Estadual Paulista “Júlio de Mesquita Filho” São Paulo State University (Unesp) Institute of Biosciences, Travessa da Rua Prof. Dr. Gilberti Moreno São Paulo
dc.description.affiliationDepartment of Anatomy Universidade Estadual Paulista “Júlio de Mesquita Filho” São Paulo State University (Unesp) Institute of Biosciences, Travessa da Rua Prof. Dr. Gilberti Moreno São Paulo
dc.description.affiliationThe Center for the Study of Venoms and Venomous Animals UNESP
dc.description.affiliationUnifai-Centro Universitário de Adamantina
dc.description.affiliationUnespGraduate Program on the General Bases of Surgery Botucatu Medical School Department of Anatomy Universidade Estadual Paulista “Júlio de Mesquita Filho” São Paulo State University (Unesp) Institute of Biosciences, Travessa da Rua Prof. Dr. Gilberti Moreno São Paulo
dc.description.affiliationUnespDepartment of Anatomy Universidade Estadual Paulista “Júlio de Mesquita Filho” São Paulo State University (Unesp) Institute of Biosciences, Travessa da Rua Prof. Dr. Gilberti Moreno São Paulo
dc.description.affiliationUnespThe Center for the Study of Venoms and Venomous Animals UNESP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent834-847
dc.identifierhttp://dx.doi.org/10.1016/j.injury.2019.03.027
dc.identifier.citationInjury, v. 50, n. 4, p. 834-847, 2019.
dc.identifier.doi10.1016/j.injury.2019.03.027
dc.identifier.issn1879-0267
dc.identifier.issn0020-1383
dc.identifier.scopus2-s2.0-85063221780
dc.identifier.urihttp://hdl.handle.net/11449/188861
dc.language.isoeng
dc.relation.ispartofInjury
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectNeuromuscular interaction
dc.subjectPeripheral nerve injury
dc.subjectSuture and heterologous fibrin sealant
dc.titleHeterologous fibrin sealant potentiates axonal regeneration after peripheral nerve injury with reduction in the number of suture pointsen
dc.typeArtigo
unesp.author.lattes8697032623119186[8]
unesp.author.lattes6840524602748457[6]
unesp.author.orcid0000-0001-6040-7898[1]
unesp.author.orcid0000-0002-3749-7853[8]
unesp.author.orcid0000-0002-9855-5594[6]

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Artigos - Anatomia - IBB
Botucatu - CEVAP - Centro de Estudos de Venenos e Animais Peçonhentos