Amino Acid Supplementation Improves the Production of Extracellular Peptidases by Aspergillus Section Flavi and their Ionic Immobilization

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dc.contributor.authorGonsales Rosa-Garzon, Nathalia
dc.contributor.authorRodrigues de Siqueira, Ana Claudia
dc.contributor.authorNaomi Hirano, Viviane
dc.contributor.authorRodrigues, Andre [UNESP]
dc.contributor.authorCosta Pessela, Benevides
dc.contributor.authorCabral, Hamilton
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionCSIC
dc.date.accessioned2020-12-10T19:59:51Z
dc.date.available2020-12-10T19:59:51Z
dc.date.issued2020-01-01
dc.description.abstractBioprocess studies have been highlighted due to the importance of physiological processes and industrial applications of enzymes. The potential of peptidase production from Aspergillus section Flavi using different amino acids as a supplemental nitrogen source was investigated. A production profile revealed that amino acids had positive effects on peptidase production when compared to the control without amino acids. Optimal production (100 U/mL) was obtained with Arginine amino acid in 96 h of fermentation. Extracellular peptidase from Aspergillus section Flavi was identified in submerged bioprocesses by in situ activity. Biochemical studies revealed that the maximum activities of the enzyme extract were obtained at pH 6.5 and a temperature of 55 degrees C. The inhibition by EDTA and PMSF suggests the presence of more than one peptidase while the Ni2+ and Cu2+ had a negative influence on the enzyme activity. When the crude extract was reversibly immobilized on ionic supports, DEAE-Agarose and MANAE-Agarose the derivative showed different profiles of thermal and pH stabilities. Hence, this study revealed the basic properties and biochemical characteristics that allowed the production improvement of this class of enzyme. Moreover, with known properties stabilization and immobilization process is required to further explore its biotechnological capacities.en
dc.description.affiliationUniv Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ribeirao Preto, SP, Brazil
dc.description.affiliationSao Paulo State Univ, Dept Biochem & Microbiol, Rio Claro, SP, Brazil
dc.description.affiliationCSIC, Inst Invest Ciencias Alimentac CIAL, Campus Univ Autonoma Madrid, Madrid, Spain
dc.description.affiliationUnespSao Paulo State Univ, Dept Biochem & Microbiol, Rio Claro, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 2011/06986-0
dc.description.sponsorshipIdCAPES: 001
dc.format.extent10
dc.identifierhttp://dx.doi.org/10.1590/1678-4324-2020190127
dc.identifier.citationBrazilian Archives Of Biology And Technology. Curitiba-parana: Inst Tecnologia Parana, v. 63, 10 p., 2020.
dc.identifier.doi10.1590/1678-4324-2020190127
dc.identifier.fileS1516-89132020000100204.pdf
dc.identifier.issn1516-8913
dc.identifier.scieloS1516-89132020000100204
dc.identifier.urihttp://hdl.handle.net/11449/196904
dc.identifier.wosWOS:000535195200001
dc.language.isoeng
dc.publisherInst Tecnologia Parana
dc.relation.ispartofBrazilian Archives Of Biology And Technology
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectfilamentous fungi
dc.subjectimmobilization
dc.subjectMANAE-agarose
dc.subjectsubmerged bioprocess
dc.subjectprotease
dc.titleAmino Acid Supplementation Improves the Production of Extracellular Peptidases by Aspergillus Section Flavi and their Ionic Immobilizationen
dc.typeArtigo
dcterms.rightsHolderInst Tecnologia Parana
unesp.author.orcid0000-0001-9240-2397[3]
unesp.author.orcid0000-0002-4164-9362[4]
unesp.author.orcid0000-0002-7365-1694[6]

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