Pharmacological characterization of cnidarian extracts from the Caribbean Sea: Evaluation of anti-snake venom and antitumor properties

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dc.contributor.authorOliveira, Cláudia S.
dc.contributor.authorCaldeira, Cleópatra A.S.
dc.contributor.authorDiniz-Sousa, Rafaela
dc.contributor.authorRomero, Dolores L.
dc.contributor.authorMarcussi, Silvana
dc.contributor.authorMoura, Laura A.
dc.contributor.authorFuly, André L.
dc.contributor.authorde Carvalho, Cicília [UNESP]
dc.contributor.authorCavalcante, Walter L.G. [UNESP]
dc.contributor.authorGallacci, Márcia [UNESP]
dc.contributor.authorPai, Maeli Dal [UNESP]
dc.contributor.authorZuliani, Juliana P.
dc.contributor.authorCalderon, Leonardo A.
dc.contributor.authorSoares, Andreimar M.
dc.contributor.institutionFundação Oswaldo Cruz de Rondônia (Fiocruz Rondônia)
dc.contributor.institutionBrazilian Marine Biotechnology Network (BioTecMar Network)
dc.contributor.institutionUniversidade Federal de Rondônia (UNIR)
dc.contributor.institutionFacultad de Biología
dc.contributor.institutionUniversidade Federal de Lavras (UFLA)
dc.contributor.institutionUniversidade Federal Fluminense (UFF)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionCentro Universitário São Lucas (UniSL)
dc.date.accessioned2018-12-11T17:38:18Z
dc.date.available2018-12-11T17:38:18Z
dc.date.issued2018-08-28
dc.description.abstractBackground: Cnidarians produce toxins, which are composed of different polypeptides that induce pharmacological effects of biotechnological interest, such as antitumor, antiophidic and anti-clotting activities. This study aimed to evaluate toxicological activities and potential as antitumor and antiophidic agents contained in total extracts from five cnidarians: Millepora alcicornis, Stichodactyla helianthus, Plexaura homomalla, Bartholomea annulata and Condylactis gigantea (total and body wall). Methods: The cnidarian extracts were evaluated by electrophoresis and for their phospholipase, proteolytic, hemorrhagic, coagulant, fibrinogenolytic, neuromuscular blocking, muscle-damaging, edema-inducing and cytotoxic activities. Results: All cnidarian extracts showed indirect hemolytic activity, but only S. helianthus induced direct hemolysis and neurotoxic effect. However, the hydrolysis of NBD-PC, a PLA2 substrate, was presented only by the C. gigantea (body wall) and S. helianthus. The extracts from P. homomalla and S. helianthus induced edema, while only C. gigantea and S. helianthus showed intensified myotoxic activity. The proteolytic activity upon casein and fibrinogen was presented mainly by B. annulata extract and all were unable to induce hemorrhage or fibrinogen coagulation. Cnidarian extracts were able to neutralize clotting induced by Bothrops jararacussu snake venom, except M. alcicornis. All cnidarian extracts were able to inhibit hemorrhagic activity induced by Bothrops moojeni venom. Only the C. gigantea (body wall) inhibited thrombin-induced coagulation. All cnidarian extracts showed antitumor effect against Jurkat cells, of which C. gigantea (body wall) and S. helianthus were the most active; however, only C. gigantea (body wall) and M. alcicornis were active against B16F10 cells. Conclusion: The cnidarian extracts analyzed showed relevant in vitro inhibitory potential over the activities induced by Bothrops venoms; these results may contribute to elucidate the possible mechanisms of interaction between cnidarian extracts and snake venoms.en
dc.description.affiliationCentro de Estudos de Biomoléculas Aplicadas a Saúde (CEBio) Fundação Oswaldo Cruz de Rondônia (Fiocruz Rondônia)
dc.description.affiliationBrazilian Marine Biotechnology Network (BioTecMar Network)
dc.description.affiliationUniversidade Federal de Rondônia (UNIR) Departamento de Medicina
dc.description.affiliationUniversidad de La Habana Centro de Estudios de Proteínas Facultad de Biología
dc.description.affiliationUniversidade Federal de Lavras (UFLA) Departamento de Química
dc.description.affiliationUniversidade Federal Fluminense (UFF) Departamento de Biologia Celular e Molecular (GCM) Instituto de Biologia
dc.description.affiliationUniversidade Estadual Paulista (UNESP) Departamento de Farmacologia Instituto de Biociências
dc.description.affiliationUniversidade Federal de Minas Gerais (UFMG) Instituto de Ciências Biológicas Departamento de Farmacologia
dc.description.affiliationUniversidade Estadual Paulista (UNESP) Departamento de Morfologia Instituto de Biociências
dc.description.affiliationCentro Universitário São Lucas (UniSL)
dc.description.affiliationUnespUniversidade Estadual Paulista (UNESP) Departamento de Farmacologia Instituto de Biociências
dc.description.affiliationUnespUniversidade Estadual Paulista (UNESP) Departamento de Morfologia Instituto de Biociências
dc.identifierhttp://dx.doi.org/10.1186/s40409-018-0161-z
dc.identifier.citationJournal of Venomous Animals and Toxins Including Tropical Diseases, v. 24, n. 1, 2018.
dc.identifier.doi10.1186/s40409-018-0161-z
dc.identifier.fileS1678-91992018000100313.pdf
dc.identifier.issn1678-9199
dc.identifier.issn1678-9180
dc.identifier.lattes6171323341671821
dc.identifier.lattes9353490382598257
dc.identifier.scieloS1678-91992018000100313
dc.identifier.scopus2-s2.0-85052515898
dc.identifier.urihttp://hdl.handle.net/11449/180136
dc.language.isoeng
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseases
dc.relation.ispartofsjr0,573
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAntiophidic
dc.subjectAntitumor
dc.subjectBioprospection
dc.subjectCaribbean sea cnidarians
dc.subjectNatural products
dc.titlePharmacological characterization of cnidarian extracts from the Caribbean Sea: Evaluation of anti-snake venom and antitumor propertiesen
dc.typeArtigo
unesp.author.lattes6171323341671821
unesp.author.lattes9353490382598257

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