Production of the First Effective Hyperimmune Equine Serum Antivenom against Africanized Bees

Santos, Keity Souza; Stephano, Marco Antonio; Marcelino, Jose Roberto; Resende Ferreira, Virginia Maria; Rocha, Thalita; Caricati, Celso; Higashi, Hisako Gondo; Moro, Ana Maria; Kalil, Jorge Elias; Malaspina, Osmar [UNESP]; Morato Castro, Fabio Fernandes; Palma, Mario Sergio [UNESP]

Production of the First Effective Hyperimmune Equine Serum Antivenom against Africanized Bees

Arquivos

WOS000327254700167.pdf (795.45 KB)

Data

2013-11-13

Autores

Santos, Keity Souza

Stephano, Marco Antonio

Marcelino, Jose Roberto

Resende Ferreira, Virginia Maria

Rocha, Thalita

Caricati, Celso

Higashi, Hisako Gondo

Moro, Ana Maria

Kalil, Jorge Elias

Malaspina, Osmar

Editor

Public Library Science

Tipo

Artigo

Direito de acesso

Acesso aberto

Resumo

Victims of massive bee attacks become extremely ill, presenting symptoms ranging from dizziness and headache to acute renal failure and multiple organ failure that can lead to death. Previous attempts to develop specific antivenom to treat these victims have been unsuccessful. We herein report a F(ab)'(2)-based antivenom raised in horse as a potential new treatment for victims of multiple bee stings. The final product contains high specific IgG titers and is effective in neutralizing toxic effects, such as hemolysis, cytotoxicity and myotoxicity. The assessment of neutralization was revised and hemolysis, the primary toxic effect of these stings, was fully neutralized in vivo for the first time.