Effects of muscarinic receptor antagonists on acetylcholine-induced contractions of jejunal smooth muscle in horses

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dc.contributor.authorTeixeira Neto, Francisco José [UNESP]
dc.contributor.authorMcDonell, W. N.
dc.contributor.authorBlack, W. D.
dc.contributor.authorHarris, W.
dc.contributor.authorGrovum, L.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Guelph
dc.date.accessioned2014-05-20T13:39:13Z
dc.date.available2014-05-20T13:39:13Z
dc.date.issued2012-08-01
dc.description.abstractTeixeira-Neto, F. J., McDonell, W. N., Black, W. D., Harris, W., Grovum, L. Effects of muscarinic receptor antagonists on acetylcholine-induced contractions of jejunal smooth muscle in horses. J. vet. Pharmacol. Therap. 35, 313-318.This study investigated the effects of a muscarinic type 1 (M1), 2 (M2), and 3 (M3) antagonists (4-DAMP, pirenzepine, and methoctramine, respectively) on acetylcholine (Ach)-induced contractions of longitudinal jejunal muscle strips of horses. Strips were irrigated with Krebs-Henseleit solution gassed with 95% O2 and 5% CO2, and the developed tension in response to Ach was recorded before and after incubation with increasing concentrations of 4-DAMP (10-810-6 m), pirenzepine (10-610-4 m), and methoctramine (10-510-3 m). When competitive antagonism was characterized, the affinity constant (pA2) was calculated by Schild plots. A parallel rightward shift in the concentrationresponse curves was observed after 4-DAMP and pirenzepine. Methoctramine presented a dual effect on the concentrationresponse curves: lower concentrations induced a parallel rightward shift without altering the maximum intensity of contraction (Emax), while the highest concentration increased slope of the concentrationresponse curve and increased Emax. The pA2 for 4-DAMP and pirenzepine was 9.18 and 7.13, respectively. Acetylcholine-induced contractions of longitudinal jejunal smooth muscle are mediated mainly via M3 receptors. The complex role of M2 receptors in jejunal smooth muscle contractions was evident because methoctramine potentiated the contractile response to higher doses of Ach.en
dc.description.affiliationUniv Estadual Paulista, Fac Med Vet & Zootecnia, Dept Cirurgia & Anestesiol Vet, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniv Guelph, Ontario Vet Coll, Dept Clin Studies, Guelph, on N1G 2W1, Canada
dc.description.affiliationUniv Guelph, Ontario Vet Coll, Dept Biomed Sci, Guelph, on N1G 2W1, Canada
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med Vet & Zootecnia, Dept Cirurgia & Anestesiol Vet, BR-18618000 Botucatu, SP, Brazil
dc.description.sponsorshipOntario Ministry of Agriculture, Food, and Rural Affairs, Canada
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent313-318
dc.identifierhttp://dx.doi.org/10.1111/j.1365-2885.2011.01330.x
dc.identifier.citationJournal of Veterinary Pharmacology and Therapeutics. Hoboken: Wiley-blackwell, v. 35, n. 4, p. 313-318, 2012.
dc.identifier.doi10.1111/j.1365-2885.2011.01330.x
dc.identifier.issn0140-7783
dc.identifier.urihttp://hdl.handle.net/11449/13599
dc.identifier.wosWOS:000306006100001
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Veterinary Pharmacology and Therapeutics
dc.relation.ispartofjcr1.441
dc.relation.ispartofsjr0,700
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleEffects of muscarinic receptor antagonists on acetylcholine-induced contractions of jejunal smooth muscle in horsesen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-blackwell
unesp.author.orcid0000-0002-3002-8093[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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Artigos - Cirurgia e Anestesiologia Veterinária - FMVZ