Annexin A1 Regulates NLRP3 Inflammasome Activation and Modifies Lipid Release Profile in Isolated Peritoneal Macrophages
dc.contributor.author | Sanches, Jose Marcos | |
dc.contributor.author | Branco, Laura Migliari | |
dc.contributor.author | Bueno Duarte, Gustavo Henrique | |
dc.contributor.author | Oliani, Sonia Maria [UNESP] | |
dc.contributor.author | Bortoluci, Karina Ramalho | |
dc.contributor.author | Moreira, Vanessa | |
dc.contributor.author | Gil, Cristiane Damas [UNESP] | |
dc.contributor.institution | Universidade Federal de São Paulo (UNIFESP) | |
dc.contributor.institution | Univ Oeste Paulista | |
dc.contributor.institution | Universidade Estadual de Campinas (UNICAMP) | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2020-12-11T19:10:17Z | |
dc.date.available | 2020-12-11T19:10:17Z | |
dc.date.issued | 2020-04-01 | |
dc.description.abstract | Annexin A1 (AnxA1) is a potent anti-inflammatory protein that downregulates proinflammatory cytokine release. This study evaluated the role of AnxA1 in the regulation of NLRP3 inflammasome activation and lipid release by starch-elicited murine peritoneal macrophages. C57bl/6 wild-type (WT) and AnxA1-null (AnxA1(-/-)) mice received an intraperitoneal injection of 1.5% starch solution for macrophage recruitment. NLRP3 was activated by priming cells with lipopolysaccharide for 3 h, followed by nigericin (1 h) or ATP (30 min) incubation. As expected, nigericin and ATP administration decreased elicited peritoneal macrophage viability and induced IL-1 beta release, more pronounced in the AnxA1(-/-) cells than in the control peritoneal macrophages. In addition, nigericin-activated AnxA1(-/-) macrophages showed increased levels of NLRP3, while points of co-localization of the AnxA1 protein and NLRP3 inflammasome were detected in WT cells, as demonstrated by ultrastructural analysis. The lipidomic analysis showed a pronounced release of prostaglandins in nigericin-stimulated WT peritoneal macrophages, while ceramides were detected in AnxA1(-/-) cell supernatants. Different eicosanoid profiles were detected for both genotypes, and our results suggest that endogenous AnxA1 regulates the NLRP3-derived IL-1 beta and lipid mediator release in macrophages. | en |
dc.description.affiliation | Univ Fed Sao Paulo, Dept Morfol & Genet, BR-04023900 Sao Paulo, Brazil | |
dc.description.affiliation | Univ Oeste Paulista, Fac Med, BR-11410980 Guaruja, SP, Brazil | |
dc.description.affiliation | Univ Fed Sao Paulo, Dept Ciencias Biol, BR-04044010 Sao Paulo, Brazil | |
dc.description.affiliation | Univ Fed Sao Paulo, Ctr Terapia Celular & Mol, BR-04044010 Sao Paulo, Brazil | |
dc.description.affiliation | Univ Estadual Campinas, Inst Quim, BR-13083862 Campinas, SP, Brazil | |
dc.description.affiliation | Univ Estadual Paulista, Programa Posgrad Biociencias, Inst Biociencias Letras & Ciencias Exatas IBILCE, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil | |
dc.description.affiliation | Univ Fed Sao Paulo, Dept Farmacol, BR-04044020 Sao Paulo, Brazil | |
dc.description.affiliationUnesp | Univ Estadual Paulista, Programa Posgrad Biociencias, Inst Biociencias Letras & Ciencias Exatas IBILCE, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
dc.description.sponsorshipId | FAPESP: 2016/02012-4 | |
dc.description.sponsorshipId | FAPESP: 2017/26872-5 | |
dc.description.sponsorshipId | CAPES: 001 | |
dc.format.extent | 15 | |
dc.identifier | http://dx.doi.org/10.3390/cells9040926 | |
dc.identifier.citation | Cells. Basel: Mdpi, v. 9, n. 4, 15 p., 2020. | |
dc.identifier.doi | 10.3390/cells9040926 | |
dc.identifier.uri | http://hdl.handle.net/11449/197794 | |
dc.identifier.wos | WOS:000535559500137 | |
dc.language.iso | eng | |
dc.publisher | Mdpi | |
dc.relation.ispartof | Cells | |
dc.source | Web of Science | |
dc.subject | inflammation | |
dc.subject | nigericin | |
dc.subject | pyroptosis | |
dc.subject | mass spectrometry | |
dc.subject | lipidomics | |
dc.title | Annexin A1 Regulates NLRP3 Inflammasome Activation and Modifies Lipid Release Profile in Isolated Peritoneal Macrophages | en |
dc.type | Artigo | |
dcterms.rightsHolder | Mdpi | |
unesp.author.orcid | 0000-0003-0918-2130[4] | |
unesp.author.orcid | 0000-0001-5579-3069[6] | |
unesp.author.orcid | 0000-0001-6979-4126[7] |