Effect of roscovitine and cycloheximide on ultrastructure of sheep oocytes

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dc.contributor.authorCrocomo, Letícia Ferrari [UNESP]
dc.contributor.authorFilho, Wolff Camargo Marques [UNESP]
dc.contributor.authorSudano, Mateus José [UNESP]
dc.contributor.authorPaschoal, Daniela Martins [UNESP]
dc.contributor.authorLandim-Alvarenga, Fernanda da Cruz [UNESP]
dc.contributor.authorBicudo, Sony Dimas [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:27:29Z
dc.date.available2014-05-27T11:27:29Z
dc.date.issued2013-01-01
dc.description.abstractIt is believed the temporary meiosis arrest with roscovitine or cycloheximide may improve the in vitro developmental competence of oocytes in different animal species. However, little is known about the effects of these inhibitors on ultrastructure of ovines cumulus-oocyte complexes (COCs). The aim of this study was to evaluate the progression of cytoplasmic maturation and the ultrastructural changes in sheep COCs exposed to roscovitine or cycloheximide, at acceptable concentrations. COCs were in vitro cultured for 24. h in maturation medium (control group) containing 100 μM roscovitine or 1 μg/mL cycloheximide (treatment groups). After this time, some COCs were cultured for further 22. h in inhibitor-free medium. The ultrastructure organization of COCs was evaluated by transmission electron microscopy before (immature group) and after in vitro culture for 24 and 46. h. As expected, signs of immaturity and maturity were observed in immature and control groups, respectively. In treatment with roscovitine, there were cumulus cells degeneration, swelling of mitochondrias, few cortical granules and many vesicles with electron-dense material. However, in cycloheximide treatment there were not signs of degeneration or cellular senescence. Metabolic units and mitochondrial pleomorphism were found in all experimental groups. These evidences demonstrate that roscovitine promoted irreversible ultrastructural changes while cycloheximide did not affect the cytoplasmic maturation. However, the implications on embryo development are still unclear. © 2012 Elsevier B.V.en
dc.description.affiliationDepartment of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science São Paulo State University, UNESP, Distrito de Rubião Junior s/no, 18603-970, Botucatu, SP
dc.description.affiliationUnespDepartment of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science São Paulo State University, UNESP, Distrito de Rubião Junior s/no, 18603-970, Botucatu, SP
dc.format.extent156-162
dc.identifierhttp://dx.doi.org/10.1016/j.smallrumres.2012.07.006
dc.identifier.citationSmall Ruminant Research, v. 109, n. 2-3, p. 156-162, 2013.
dc.identifier.doi10.1016/j.smallrumres.2012.07.006
dc.identifier.file2-s2.0-84871476800.pdf
dc.identifier.issn0921-4488
dc.identifier.lattes8134606270913860
dc.identifier.scopus2-s2.0-84871476800
dc.identifier.urihttp://hdl.handle.net/11449/74196
dc.identifier.wosWOS:000313844900014
dc.language.isoeng
dc.relation.ispartofSmall Ruminant Research
dc.relation.ispartofjcr0.974
dc.relation.ispartofsjr0,485
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCytoplasmic maturation
dc.subjectMeiosis inhibitors
dc.subjectOocyte
dc.subjectOvine
dc.subjectAnimalia
dc.subjectOvis
dc.subjectOvis aries
dc.titleEffect of roscovitine and cycloheximide on ultrastructure of sheep oocytesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.lattes8134606270913860
unesp.author.orcid0000-0002-6174-3153[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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