Acute green tea intake attenuates circulating microRNA expression induced by a high-fat, high-saturated meal in obese women: A randomized crossover study

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dc.contributor.authorBastos, Rodrigo V.S. [UNESP]
dc.contributor.authorDorna, Mariana S. [UNESP]
dc.contributor.authorChiuso-Minicucci, Fernanda [UNESP]
dc.contributor.authorFelix, Tainara F. [UNESP]
dc.contributor.authorFernandes, Ana A.H. [UNESP]
dc.contributor.authorAzevedo, Paula S. [UNESP]
dc.contributor.authorFranco, Estefânia T. [UNESP]
dc.contributor.authorPolegato, Bertha F. [UNESP]
dc.contributor.authorRogero, Marcelo M.
dc.contributor.authorMota, Gustavo A.F. [UNESP]
dc.contributor.authorQuintanilha, Bruna J.
dc.contributor.authorPaiva, Sergio A.R. [UNESP]
dc.contributor.authorZornoff, Leonardo A.M. [UNESP]
dc.contributor.authorReis, Patricia P. [UNESP]
dc.contributor.authorMinicucci, Marcos F. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2023-07-29T13:30:46Z
dc.date.available2023-07-29T13:30:46Z
dc.date.issued2023-02-01
dc.description.abstractThe objective of this study was to assess whether acute green tea (GT) supplementation attenuates inflammatory and oxidative stress biomarkers induced by high-fat, high-saturated (HFHS) meals in obese women, and to assess its ability to modulate circulating microRNA (miRNA) expression. This was a randomized, double-blind, crossover study. The study included obese women over 18 years old who had no comorbidities. In the first moment, patients were instructed to take 2 capsules of placebo or GT (738 mg) at 10:00 p.m. and to fast overnight. The next morning, a blood sample was collected, and an HFHS meal was offered to the patients. Another blood sample was collected 5 hours after the meal. In the second moment, patients who received placebo in the first moment now received the GT and vice-versa. Serum inflammatory and oxidative stress biomarkers were measured, and circulating levels of miRNA were evaluated. Fifteen women with mean age of 35.5±9.9 years were included in the final analysis. There was no difference regarding inflammatory and oxidative stress biomarkers. However, patients who consumed GT had lower circulating expression of 62 miRNAs compared with patients who did not consume GT. Predictive analysis of target genes showed 1,757 targets regulated by the 62 miRNAs. Notably, 5 miRNAs (miR-1297, miR-192-5p, miR-373-3p, miR-595 and miR-1266-5p) regulate genes associated with TGF-beta, CARM1, RSK, and BMP pathways. Our study showed that GT inhibited the expression of miRNAs induced by HFHS meal intake. These results shed light on the mechanisms involved in the beneficial effects of GT ingestion.en
dc.description.affiliationInternal Medicine Department Botucatu Medical School São Paulo State University, Botucatu
dc.description.affiliationExperimental Research Unit Botucatu Medical School São Paulo State University, Botucatu
dc.description.affiliationChemistry and Biochemistry Department Institute of Biosciences São Paulo State University, Botucatu
dc.description.affiliationDepartment of Nutrition School of Public Health USP - University of São Paulo, Sao Paulo
dc.description.affiliationDepartment of Surgery and Orthopedics Botucatu Medical School São Paulo State University, Botucatu
dc.description.affiliationUnespInternal Medicine Department Botucatu Medical School São Paulo State University, Botucatu
dc.description.affiliationUnespExperimental Research Unit Botucatu Medical School São Paulo State University, Botucatu
dc.description.affiliationUnespChemistry and Biochemistry Department Institute of Biosciences São Paulo State University, Botucatu
dc.description.affiliationUnespDepartment of Surgery and Orthopedics Botucatu Medical School São Paulo State University, Botucatu
dc.identifierhttp://dx.doi.org/10.1016/j.jnutbio.2022.109203
dc.identifier.citationJournal of Nutritional Biochemistry, v. 112.
dc.identifier.doi10.1016/j.jnutbio.2022.109203
dc.identifier.issn1873-4847
dc.identifier.issn0955-2863
dc.identifier.scopus2-s2.0-85142879203
dc.identifier.urihttp://hdl.handle.net/11449/247965
dc.language.isoeng
dc.relation.ispartofJournal of Nutritional Biochemistry
dc.sourceScopus
dc.subjectCamellia sinensis
dc.subjecthigh-fat meal
dc.subjectinflammatory process
dc.subjectmicroRNA
dc.subjectoxidative stress
dc.titleAcute green tea intake attenuates circulating microRNA expression induced by a high-fat, high-saturated meal in obese women: A randomized crossover studyen
dc.typeArtigo
unesp.author.orcid0000-0002-6705-5749[1]
unesp.author.orcid0000-0003-3155-9088[4]
unesp.author.orcid0000-0002-2875-9532[8]
unesp.author.orcid0000-0003-0517-1645[9]
unesp.author.orcid0000-0001-8103-3338[10]
unesp.author.orcid0000-0003-4412-1990[12]
unesp.author.orcid0000-0003-3775-3797 0000-0003-3775-3797[14]
unesp.author.orcid0000-0002-5980-4367[15]

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