Fibroblast Growth Factor-2 Regulation of Sprouty and NR4A Genes in Bovine Ovarian Granulosa Cells

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dc.contributor.authorJiang, Z. L.
dc.contributor.authorRipamonte, P. [UNESP]
dc.contributor.authorBuratini, J. [UNESP]
dc.contributor.authorPortela, V. M.
dc.contributor.authorPrice, C. A.
dc.contributor.institutionUniv Montreal
dc.contributor.institutionNW A&F Univ
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:32:09Z
dc.date.available2014-05-20T15:32:09Z
dc.date.issued2011-07-01
dc.description.abstractFibroblast growth factors (FGFs) alter ovarian function, at least in part by inhibiting steroid hormone secretion and affecting survival of granulosa cells. The mechanism of action of FGFs in ovarian follicle cells is largely unknown; in the present study we identified the major pathways used by FGF2 in non-luteinizing granulosa cells cultured under serum-free conditions. FGF2 increased abundance of mRNA encoding SPRY1, 2, and 4, but not SPRY3. Common pathways employed by FGF2 in the regulation of SPRY1, 2, and 4, as demonstrated by immunoblot and inhibitor studies, included ERK1/2 and Akt signaling. In contrast, PKC activation was necessary for FGF2-stimulated expression of SPRY1 and 4, but not for SPRY2. Intracellular calcium flux is critical and sufficient for SPRY2 expression, but not for SPRY1 and 4. We also identified the orphan nuclear receptor NR4A1 as a potential early response gene in FGF2 signaling, whose expression, like that of SPRY2, is critically dependent on calcium signaling. Together, these data identify FGF2-target genes in follicular granulosa cells, and demonstrate alternative pathway use for the differential control of SPRY genes. J. Cell. Physiol. 226: 1820-1827, 2011. (C) 2010 Wiley-Liss, Inc.en
dc.description.affiliationUniv Montreal, Fac Med Vet, CRRA, St Hyacinthe, PQ J2S 7C6, Canada
dc.description.affiliationNW A&F Univ, Coll Anim Sci & Technol, Yangling, Shaanxi, Peoples R China
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Dept Fisiol, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Dept Fisiol, São Paulo, Brazil
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada (NSERC)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdNSERC, Canada: 106389-05
dc.description.sponsorshipIdFAPESP: 06/56354-1
dc.description.sponsorshipIdFAPESP: 06/56374-2
dc.format.extent1820-1827
dc.identifierhttp://dx.doi.org/10.1002/jcp.22509
dc.identifier.citationJournal of Cellular Physiology. Malden: Wiley-blackwell, v. 226, n. 7, p. 1820-1827, 2011.
dc.identifier.doi10.1002/jcp.22509
dc.identifier.issn0021-9541
dc.identifier.urihttp://hdl.handle.net/11449/41133
dc.identifier.wosWOS:000290519900015
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Cellular Physiology
dc.relation.ispartofjcr3.923
dc.relation.ispartofsjr1,641
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleFibroblast Growth Factor-2 Regulation of Sprouty and NR4A Genes in Bovine Ovarian Granulosa Cellsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-blackwell
unesp.author.orcid0000-0002-6727-8280[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Fisiologia - IBB