Meiotic inhibition of bovine oocytes in medium supplemented with a serum replacer and hormones: effects on meiosis progression and developmental capacity

dc.contributor.authorSa Barretto, Leticia Siqueira [UNESP]
dc.contributor.authorDias Caiado Castro, Viviane Sgobbi [UNESP]
dc.contributor.authorGarcia, Joaquim Mansano [UNESP]
dc.contributor.authorMingoti, Gisele Zoccal [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:15:29Z
dc.date.available2014-05-20T13:15:29Z
dc.date.issued2011-05-01
dc.description.abstractAiming to improve the developmental competence of bovine oocytes during meiotic block, this study evaluated the effects of a serum replacer (Knockout SR(R)) and hormones (gonadotropins and estradiol) supplementation of prematuration medium (TCM119 with 0.5 mM IBMX [IBMX group] or 25 mu M roscovitine [ROSC group]) on the kinetics of oocyte nuclear maturation and embryo development. Most IBMX and ROSC oocytes prematured for 8 h culture remained in the GV stage (70.3% and 73.1%, respectively; p > 0.05) similar to Control 8 h (63.5%) and to control immature oocytes (Control 0 h, 92.5%). After prematuration for 16 h, no oocytes remained in the GV stage at similar rates to those recently aspirated (p < 0.05); GV rates in ROSC (32.4%) were higher (p < 0.05) than in the Control 16 h group (8.6%), but similar (p > 0.05) to IBMX (9.7%). After in vitro maturation (IMV) for 24 h, metaphase II (MII) rates for oocytes prematured during 8 h were similar (p > 0.05) between control and treatments (65.0-71.7%). Similarly, MII rates oocytes prematured during 16 h were similar (p > 0.05) between all groups (45.9-60.4%). Cleavage rates (67.8-78.2%), embryonic development in day-7 (25.0-35.6%) and hatching rates in day-8 (2.5-11.3%) oocytes blocked during 8 h were similar for all groups (p > 0.05). Results indicate that addition of Knockout SR(R) and hormones to meiotic block culture with IBMX and roscovitine negatively affected meiotic arrest, but did not impair oocyte nuclear maturation and acquisition of developmental competence.en
dc.description.affiliationUniv Estadual Paulista, Dept Anim Hlth, Sch Vet Med, BR-16050680 São Paulo, Brazil
dc.description.affiliationUNESP, Dept Anim Reprod, Sch Agr & Vet Sci, BR-14884900 Jaboticabal, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Anim Hlth, Sch Vet Med, BR-16050680 São Paulo, Brazil
dc.description.affiliationUnespUNESP, Dept Anim Reprod, Sch Agr & Vet Sci, BR-14884900 Jaboticabal, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 01/06137-0
dc.format.extent107-116
dc.identifierhttp://dx.doi.org/10.1017/S096719941000016X
dc.identifier.citationZygote. New York: Cambridge Univ Press, v. 19, n. 2, p. 107-116, 2011.
dc.identifier.doi10.1017/S096719941000016X
dc.identifier.fileWOS000289786900002.pdf
dc.identifier.issn0967-1994
dc.identifier.lattes2251116139872527
dc.identifier.orcid0000-0002-3059-4458
dc.identifier.urihttp://hdl.handle.net/11449/2608
dc.identifier.wosWOS:000289786900002
dc.language.isoeng
dc.publisherCambridge University Press
dc.relation.ispartofZygote
dc.relation.ispartofjcr1.114
dc.relation.ispartofsjr0,387
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCattleen
dc.subjectIBMXen
dc.subjectKnockout serum replaceren
dc.subjectIn vitro maturationen
dc.subjectMeiotic arresten
dc.subjectRoscovitineen
dc.titleMeiotic inhibition of bovine oocytes in medium supplemented with a serum replacer and hormones: effects on meiosis progression and developmental capacityen
dc.typeArtigo
dcterms.licensehttp://journals.cambridge.org/action/displaySpecialPage?pageId=4676#
dcterms.rightsHolderCambridge Univ Press
unesp.author.lattes2251116139872527
unesp.author.orcid0000-0002-3059-4458[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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