Sperm viability, serological, molecular, and modified seminal plasma agglutination tests in the diagnosis of Leptospira in the semen and serum of bovine bulls

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dc.contributor.authorMaiolino, Sérgio Ricardo [UNESP]
dc.contributor.authorCortez, Adriana
dc.contributor.authorLangoni, Helio [UNESP]
dc.contributor.authorGiuffrida, Rogério
dc.contributor.authordos Santos, Joeleni Rosa [UNESP]
dc.contributor.authorde Nardi Júnior, Geraldo
dc.contributor.authorLara, Gustavo Henrique Batista [UNESP]
dc.contributor.authorMotta, Rodrigo Garcia [UNESP]
dc.contributor.authorChacur, Marcelo George Mungai
dc.contributor.authorMonteiro, Fábio Morato
dc.contributor.authorHeinemann, Marcos Bryan
dc.contributor.authorde Souza Filho, Antônio Francisco
dc.contributor.authorde Souza Araújo Martins, Lorrayne [UNESP]
dc.contributor.authorBello, Thaís Spessotto [UNESP]
dc.contributor.authorRibeiro, Márcio Garcia [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionSanto Amaro University - UNISA
dc.contributor.institutionUniversity of Oeste Paulista
dc.contributor.institutionTechnology Faculty - FATEC
dc.contributor.institutionInstitute of Animal Science
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2022-05-01T08:44:43Z
dc.date.available2022-05-01T08:44:43Z
dc.date.issued2021-01-01
dc.description.abstractThe present study investigated the serum microscopic agglutination test (MAT) among 203 bovine bulls with reproduction by natural means, without apparent signs of orchitis or inflammation of accessory reproductive glands. Simultaneously, the semen of all bulls was subjected to sperm viability analysis and PCR based on the 16S rRNA gene. PCR-positive results of semen samples were confirmed by sequencing. A modified seminal plasma agglutination (MSPA) test, replacing the blood serum of all bulls in the MAT with seminal plasma was performed as well. Eight (8/203 = 3.9%) semen samples from bulls were considered nonviable (necrospermia and azoospermia) without relation to the PCR diagnosis. No agglutinin titers were identified in MSPA test. A high frequency (132/203 = 65%) of leptospiral agglutinin titers was identified in the MAT, particularly for the Sejroe serogroup (Hardjo CTG, 100/203 = 49.3%; Wolffi 74/203 = 36.4%; Guaricura 72/203 = 35.5%; and Hardjoprajitno 56/203 = 27.6%). Three (3/203 = 1.5%) semen samples of bulls were positive in the PCR, but these results were not confirmed by sequencing. The high frequency of serovars from the Sejroe serogroup typically adapted to bovines indicates the need for measures for the prophylaxis/control of the pathogen on the sampled farms. Discrepancies among the MAT, sperm viability, and molecular detection of leptospires in semen highlight the need for a combination of methods to diagnose leptospirosis in bovine bulls. To our knowledge, modified seminal plasma agglutination is described for the first time here to investigate anti-Leptospira antibodies produced locally in the genital tract in the diagnosis of bovine leptospirosis among bulls that reproduce by natural means.en
dc.description.affiliationDepartment of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine an Animal Science São Paulo State University - UNESP
dc.description.affiliationSanto Amaro University - UNISA
dc.description.affiliationFaculty of Veterinary Medicine University of Oeste Paulista
dc.description.affiliationTechnology Faculty - FATEC
dc.description.affiliationAPTA Beef Cattle Center Institute of Animal Science
dc.description.affiliationUniversity of São Paulo - USP
dc.description.affiliationUnespDepartment of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine an Animal Science São Paulo State University - UNESP
dc.identifierhttp://dx.doi.org/10.1007/s42770-021-00562-8
dc.identifier.citationBrazilian Journal of Microbiology.
dc.identifier.doi10.1007/s42770-021-00562-8
dc.identifier.issn1678-4405
dc.identifier.issn1517-8382
dc.identifier.scopus2-s2.0-85113803639
dc.identifier.urihttp://hdl.handle.net/11449/233448
dc.language.isoeng
dc.relation.ispartofBrazilian Journal of Microbiology
dc.sourceScopus
dc.subjectBull leptospirosis
dc.subjectMolecular diagnosis
dc.subjectReproduction by natural means
dc.subjectSeroprevalence
dc.titleSperm viability, serological, molecular, and modified seminal plasma agglutination tests in the diagnosis of Leptospira in the semen and serum of bovine bullsen
dc.typeArtigo
unesp.author.orcid0000-0003-2682-9389[15]

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