Determinação de GMP e CMP* no leite por métodos espectrofotométrico (ANSM) e cromatográfico (HPLC) - parâmetros metodológicos (*glicomacropeptídeo e caseinomacropeptídeo)

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dc.contributor.authorPrata, Luiz Francisco [UNESP]
dc.contributor.authorPrata, Camila Barbieri [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:26:57Z
dc.date.available2014-05-27T11:26:57Z
dc.date.issued2012-08-30
dc.description.abstractGlycomacropeptide is a glycosilated fraction of bovine kappa-casein that remains soluble when milk is clotted by rennin. Determinations of milk sialic acid content are useful because its concentration reflects the amount of free GMP of milk. In normal milk these amounts are very low, 12 to 16 times lower than in sweet whey. Therefore, its determination may be applied to verify possible frauds with whey addictions, since it works as a fingerprint. With the description of a new spectrophotometric method for determination of free GMP (ANSM) occurred a simplification of procedures, being faster than others (HPLC method), without loss of accuracy. However, due to variations of glycosilation in kappa-casein between animals, during the lactation period, due to mastitis and yet due to proteolysis on milk, it was necessary to know these variations to interpret correctly the analytical results. It was analyzed 1,703 samples of producer's raw milk and 1,189 samples of processed milk (HTST and UHT). The results showed that normal milk from herd (producer's milk) have only small amounts of free GMP, with A470nm = 0.232±0.088 or 3.89±1.25 mg of sialic acid/L. The upper limit of this distribution was A = 0.496; thus every bigger value may represent a problem, being outside of normal distribution.en
dc.description.affiliationFCAV Unesp
dc.description.affiliationFCAVJ Unesp
dc.description.affiliationUnespFCAV Unesp
dc.description.affiliationUnespFCAVJ Unesp
dc.format.extent29-39
dc.identifierhttp://ojs.c3sl.ufpr.br/ojs/index.php/veterinary/article/view/16554
dc.identifier.citationArchives of Veterinary Science, v. 17, n. 2, p. 29-39, 2012.
dc.identifier.file2-s2.0-84865398299.pdf
dc.identifier.issn1517-784X
dc.identifier.lattes0105170548508734
dc.identifier.scopus2-s2.0-84865398299
dc.identifier.urihttp://hdl.handle.net/11449/73519
dc.language.isopor
dc.relation.ispartofArchives of Veterinary Science
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAnalysis
dc.subjectFraud
dc.subjectInterpretation
dc.subjectMilk proteolysis
dc.subjectSweet whey
dc.titleDeterminação de GMP e CMP* no leite por métodos espectrofotométrico (ANSM) e cromatográfico (HPLC) - parâmetros metodológicos (*glicomacropeptídeo e caseinomacropeptídeo)pt
dc.title.alternativeDetermination of GMP and CMP in milk by spectrophotometric - ANSM, and by chromatographic - HPLC, methods - methodological parametersen
dc.typeArtigo
dcterms.licensehttp://ojs.c3sl.ufpr.br/ojs2/index.php/veterinary/about/editorialPolicies#openAccessPolicy
unesp.author.lattes0105170548508734
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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Artigos - Medicina Veterinária Preventiva e Reprodução Animal - FCAV