Progesterone and vitamin D downregulate the activation of the NLRP1/NLRP3 inflammasomes and TLR4-MyD88-NF-κB pathway in monocytes from pregnant women with preeclampsia

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dc.contributor.authorMatias, Mariana Leticia [UNESP]
dc.contributor.authorRomao-Veiga, Mariana [UNESP]
dc.contributor.authorRibeiro, Vanessa Rocha [UNESP]
dc.contributor.authorNunes, Priscila Rezeck [UNESP]
dc.contributor.authorGomes, Virginia Juliani [UNESP]
dc.contributor.authorDevides, Amanda Carreira [UNESP]
dc.contributor.authorBorges, Vera Therezinha [UNESP]
dc.contributor.authorRomagnoli, Graziela Goretti
dc.contributor.authorPeracoli, Jose Carlos [UNESP]
dc.contributor.authorPeracoli, Maria Terezinha [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionOeste Paulista University (UNOESTE)
dc.date.accessioned2021-06-25T10:52:06Z
dc.date.available2021-06-25T10:52:06Z
dc.date.issued2021-04-01
dc.description.abstractThis study evaluated the in vitro modulatory effect of progesterone (PG) and vitamin D (VD) on NLRP1/NLRP3 inflammasomes and TLR4/NF-κB pathway in monocytes from pregnant women with preeclampsia (PE). Monocytes from 20 preeclamptic and 20 normotensive (NT) pregnant women, and THP-1 cells were cultured with/without hyaluronan (HA), PG, or VD to determine gene and protein expression of TLR4 receptor, phosphorylated NF-κB, IκBα, TLR4, MYD88, NF-κB, NLRP1, NLRP3, caspase-1, IL-1β, IL-18, TNF-α, and IL-10. Higher endogenous activation of inflammatory genes and higher protein expression of TLR4 and NF-κB was detected in monocytes of PE group and decreased after PG or VD treatment. Monocyte from PE stimulated with HA increased while treatment with PG or VD decreased the expression of genes and proteins related to the inflammasomes. THP-1 cells showed a similar immune response profile as monocytes from PE. These results demonstrate that PG and VD play an immunomodulatory role in monocyte activation.en
dc.description.affiliationDepartment of Gynaecology and Obstetrics Botucatu Medical School Sao Paulo State University (UNESP)
dc.description.affiliationDepartment Health Science Oeste Paulista University (UNOESTE)
dc.description.affiliationDepartment of Chemistry and Biological Sciences Institute of Biosciences Sao Paulo State University (UNESP), Botucatu
dc.description.affiliationUnespDepartment of Gynaecology and Obstetrics Botucatu Medical School Sao Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Chemistry and Biological Sciences Institute of Biosciences Sao Paulo State University (UNESP), Botucatu
dc.identifierhttp://dx.doi.org/10.1016/j.jri.2021.103286
dc.identifier.citationJournal of Reproductive Immunology, v. 144.
dc.identifier.doi10.1016/j.jri.2021.103286
dc.identifier.issn1872-7603
dc.identifier.issn0165-0378
dc.identifier.scopus2-s2.0-85100668471
dc.identifier.urihttp://hdl.handle.net/11449/207259
dc.language.isoeng
dc.relation.ispartofJournal of Reproductive Immunology
dc.sourceScopus
dc.subjectMonocytes
dc.subjectNLRP1/NLRP3 inflammasomes
dc.subjectPreeclampsia
dc.subjectProgesterone
dc.subjectVitamin D
dc.titleProgesterone and vitamin D downregulate the activation of the NLRP1/NLRP3 inflammasomes and TLR4-MyD88-NF-κB pathway in monocytes from pregnant women with preeclampsiaen
dc.typeArtigo
unesp.author.orcid0000-0002-8990-0237[2]
unesp.author.orcid0000-0002-9347-9641[7]
unesp.author.orcid0000-0002-0936-9512[10]

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Artigos - Ginecologia e Obstetrícia - FMB
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