Porphyromonas gingivalis LPS stimulation downregulates DNMT1, DNMT3a, and JMJD3 gene expression levels in human HaCaT keratinocytes

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dc.contributor.authorde Camargo Pereira, Gláucia
dc.contributor.authorGuimarães, Gustavo Narvaes
dc.contributor.authorPlanello, Aline Cristiane
dc.contributor.authorSantamaria, Mauro Pedrine [UNESP]
dc.contributor.authorde Souza, Ana Paula
dc.contributor.authorLine, Sergio Roberto
dc.contributor.authorMarques, Marcelo Rocha
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:27:27Z
dc.date.available2014-05-27T11:27:27Z
dc.date.issued2013-01-01
dc.description.abstractObjective: The role of epigenetic regulation in inflammatory diseases such as periodontitis is poorly known. The aim of this study was to assess whether Porphyromonas gingivalis lipopolysaccharide (LPS) can modulate gene expression levels of the some enzymes that promote epigenetic events in cultures of the human keratinocytes and gingival fibroblasts. In addition, the same enzymes were evaluated in gingival samples from healthy and periodontitis-affected individuals. Materials and methods: Primary gingival fibroblast and keratinocyte (HaCaT) cultures were treated with medium containing P. gingivalis LPS or P. gingivalis LPS vehicle for 24 h. After this period, cell viability was assessed by MTT test and total RNA extracted to evaluate gene expression levels of the following enzymes by qRT-PCR: DNA methyltransferase 1 (DNMT1), DNA methyltransferase 3a (DNMT3a), histone demethylases Jumonji domain containing 3 (JMJD3) and ubiquitously transcribed tetratricopeptide repeat, X chromosome (UTX). To evaluate gene expression in healthy and periodontitis-affected individuals, total RNA was extracted from biopsies of gingival tissue from healthy and periodontitis sites, and gene expression of DNMT1, DNAMT3a, JMJD3, and UTX was evaluated by qRT-PCR. Results: No significant differences were found in the gene expression analysis between healthy and periodontitis-affected gingival samples. The results showed that LPS downregulated DNMT1 (p < 0. 05), DNMT3a (p < 0. 05), and JMJD3 (p < 0. 01) gene expression in HaCaT cells, but no modulation was observed in gingival fibroblasts. Conclusion: P. gingivalis LPS exposure to human HaCaT keratinocytes downregulates gene expression of the enzymes that promote epigenetic events. Clinical relevance: The advance knowledge about epigenetic modifications caused by periodontopathogens may to possibly led to the development of new periodontal therapies. © 2012 Springer-Verlag.en
dc.description.affiliationDepartment of Morphology Division of Histology, Piracicaba Dental School State University of Campinas, Av. Limeira 901, Caixa Postal 052, CEP 13414-903 Piracicaba, São Paulo
dc.description.affiliationDepartment of Diagnostic and Surgery University Estadual Paulista (UNESP), São José dos Campos, São Paulo
dc.description.affiliationUnespDepartment of Diagnostic and Surgery University Estadual Paulista (UNESP), São José dos Campos, São Paulo
dc.format.extent1279-1285
dc.identifierhttp://dx.doi.org/10.1007/s00784-012-0816-z
dc.identifier.citationClinical Oral Investigations, v. 17, n. 4, p. 1279-1285, 2013.
dc.identifier.doi10.1007/s00784-012-0816-z
dc.identifier.issn1432-6981
dc.identifier.issn1436-3771
dc.identifier.lattes7008114923397947
dc.identifier.scopus2-s2.0-84876428659
dc.identifier.urihttp://hdl.handle.net/11449/74120
dc.identifier.wosWOS:000317866800020
dc.language.isoeng
dc.relation.ispartofClinical Oral Investigations
dc.relation.ispartofjcr2.386
dc.relation.ispartofsjr0,986
dc.relation.ispartofsjr0,986
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectEpigenetic
dc.subjectGingival fibroblasts
dc.subjectKeratinocytes
dc.subjectP. gingivalis LPS
dc.subjectPeriodontitis
dc.titlePorphyromonas gingivalis LPS stimulation downregulates DNMT1, DNMT3a, and JMJD3 gene expression levels in human HaCaT keratinocytesen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
unesp.author.lattes7008114923397947
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Ciência e Tecnologia, São José dos Campospt

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