Parthenogenetic activation of bovine oocytes using single and combined strontium, ionomycin and 6-dimethylaminopurine treatments

Página do item simplificado
dc.contributor.authorMeo, S. C.
dc.contributor.authorYamazaki, W.
dc.contributor.authorFerreira, C. R.
dc.contributor.authorPerecin, F.
dc.contributor.authorSaraiva, N. Z.
dc.contributor.authorLeal, C. L. V.
dc.contributor.authorGarcia, J. M.
dc.contributor.institutionInst Zootecnia
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:15:13Z
dc.date.available2014-05-20T13:15:13Z
dc.date.issued2007-11-01
dc.description.abstractIn vitro-matured (IVM) bovine oocytes were activated with single and combined treatments of strontium (S), ionomycin (1) and 6-DMAP (D). Using oocytes IVM for 26 h, we observed that activation altered cell cycle kinetics (faster progression, MIII arrest, or direct transition from MII to pronuclear stage) when compared to in vitro fertilization. The effect of oocyte age on early parthenogenesis was assessed in oocytes IVM for 22, 26 and 30 h. Better results in pronuclear development were obtained in treatments ISD (81.7%) at 22 h; D (66.7%), IS (63.3%), ID (73.3%) and ISD (76.7%) at 26 h; and D (86.7%), IS (85.0%) and ID (78.3%) at 30 h. Higher cleavage occurred on ISD (80.0%) at 22 h; ID (83.3%) and ISD (91.7%) at 26 h; and 1 (86.7%), IS (90.0%), ID (85.0%) and ISD (95.0%) at 30 h. More blastocysts were achieved in ID (25.0%) and ISD (18.3%) at 22h; and in ID at 26h (45.0%) and 30h (50.0%). We also observed that IS allowed higher haploid (77.4%) embryonic development, whilst ID was better for diploid (89.1%) development. It was concluded that association of S and D without I was not effective for blastocyst development; treatments using S were less influenced by oocyte age, but when S was associated with D there was a detrimental effect on aged oocytes; treatment ISD promoted higher activation and cleavage rates in young oocytes and ID protocol was the best for producing blastocysts.en
dc.description.affiliationInst Zootecnia, Ctr Genet & Reprod Anim, BR-13460000 Nova Odessa, SP, Brazil
dc.description.affiliationUNESP, FCAV, Dept Med Vet Prevent & Reprod Anim, BR-14884900 Jaboticabal, SP, Brazil
dc.description.affiliationUSP, FZEA, Dept Ciências Basicas, BR-13635900 Pirassununga, SP, Brazil
dc.description.affiliationUnespUNESP, FCAV, Dept Med Vet Prevent & Reprod Anim, BR-14884900 Jaboticabal, SP, Brazil
dc.format.extent295-306
dc.identifierhttp://dx.doi.org/10.1017/S0967199407004285
dc.identifier.citationZygote. New York: Cambridge Univ Press, v. 15, n. 4, p. 295-306, 2007.
dc.identifier.doi10.1017/S0967199407004285
dc.identifier.fileWOS000250677700002.pdf
dc.identifier.issn0967-1994
dc.identifier.lattes2251116139872527
dc.identifier.urihttp://hdl.handle.net/11449/2426
dc.identifier.wosWOS:000250677700002
dc.language.isoeng
dc.publisherCambridge University Press
dc.relation.ispartofZygote
dc.relation.ispartofjcr1.114
dc.relation.ispartofsjr0,387
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCattlept
dc.subjectchromatinpt
dc.subjectembryonic developmentpt
dc.subjectmicrotubulespt
dc.subjectoocyte activationpt
dc.titleParthenogenetic activation of bovine oocytes using single and combined strontium, ionomycin and 6-dimethylaminopurine treatmentsen
dc.typeArtigo
dcterms.licensehttp://journals.cambridge.org/action/displaySpecialPage?pageId=4676
dcterms.rightsHolderCambridge Univ Press
unesp.author.lattes2251116139872527
unesp.author.orcid0000-0003-3988-6454[3]
unesp.author.orcid0000-0003-0046-0050[1]
unesp.author.orcid0000-0003-2009-5863[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.departmentMedicina Veterinária Preventiva e Reprodução Animal - FCAVpt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000250677700002.pdf
Tamanho:
243.12 KB
Formato:
Adobe Portable Document Format
Baixar
Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias