Efeito de um quelante de cálcio, um detergente e da lecitina de soja sobre a qualidade do sêmen caprino congelado-descongelado

dc.contributor.authorBittencourt, Rodrigo Freitas [UNESP]
dc.contributor.authorRibeiro Filho, Antönio de Lisboa
dc.contributor.authorLima, Marcos Chalhoub Coelho
dc.contributor.authorAlves, Sidney Gonc̈alves Gonzalez
dc.contributor.authorVasconcelos, Marta Freitas
dc.contributor.authorBiscarde, Carmo Emanuel [UNESP]
dc.contributor.authorLeal, Luciana da Silva [UNESP]
dc.contributor.authorOba, Eunice [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal da Bahia (UFBA)
dc.contributor.institutionUniversidade de Brasília (UnB)
dc.date.accessioned2014-05-27T11:23:48Z
dc.date.available2014-05-27T11:23:48Z
dc.date.issued2008-12-01
dc.description.abstractThe aim of this paper was verifying the effect of the Equex STM Paste and EDTA addition to a Tris-egg yolk extender, on the postthaw goat sperm viability. Nine semen samples of two adult goats were collected by artificial vagina and cryopreserved. It was also objective of this study, to evaluate the utilization of a soybean lecithin based commercial extender (Bioexcell® - IMV, L'Aigle, French) for the goat semen freezing. They were formed five experimental groups: TRIS; TRIS+EDTA; TRIS+EQUEX; TRIS+EDTA+EQUEX e Bioexcell. After evaluation, the semen was diluted in the five extenders and packed in 0.25mL straws with 100 million of motile spermatozoa. The samples were cooled at 0,46°C/min to 5°C, submitted at 75min of equilibration time and frozen in liquid nitrogen vapour. The thawing was accomplished in 37°C water bath for 50s. There were no differences (P>0,05) on the means of post-thaw total and progressive sperm motility among the groups TRIS, TRIS+EQUEX and TRIS+EQUEX+EDTA. The Bioexcell group obtained the least (P<0,05) percentage of post-thawing total and progressive sperm motility. After the thermotolerance test, it was observed the greatest (P<0,05) rates of total and progressive sperm motility in the Equex STM groups (TRIS+EQUEX and TRIS+EQUEX+EDTA). Thus, it can be affirmed that the Equex addition promotes better maintenance rates in the pos-thaw sperm viability, when compared with the extenders that did not contain it.en
dc.description.affiliationFaculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista, Botucatu-SP
dc.description.affiliationEscola de Medicina Veterinária Universidade Federal da Bahia, Salvador-BA
dc.description.affiliationFaculdade de Medicina Veterinária Universidade de Brasília, Brasília-DF
dc.description.affiliationUnespFaculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista, Botucatu-SP
dc.format.extent305-312
dc.identifierhttp://www.revistas.usp.br/bjvras/article/view/26690
dc.identifier.citationBrazilian Journal of Veterinary Research and Animal Science, v. 45, n. 4, p. 305-312, 2008.
dc.identifier.file2-s2.0-78149372698.pdf
dc.identifier.issn1413-9596
dc.identifier.issn1678-4456
dc.identifier.lattes9224380586557664
dc.identifier.scopus2-s2.0-78149372698
dc.identifier.urihttp://hdl.handle.net/11449/70812
dc.language.isopor
dc.relation.ispartofBrazilian Journal of Veterinary Research and Animal Science
dc.relation.ispartofsjr0,225
dc.relation.ispartofsjr0,225
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCryopreservation
dc.subjectExtender
dc.subjectGoat
dc.subjectSemen
dc.subjectCapra hircus
dc.subjectGlycine max
dc.titleEfeito de um quelante de cálcio, um detergente e da lecitina de soja sobre a qualidade do sêmen caprino congelado-descongeladopt
dc.title.alternativeEffects of a calcium chelator, a detergent and the soybean lecithin on the quality of the frozen-thawed goat semenen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/bjvras/paboutj.htm
unesp.author.lattes9224380586557664
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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