Genotoxic evaluation of the antimalarial drugs artemisinin and artesunate in human HepG2 cells and effects on CASP3 and SOD1 gene expressions

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dc.contributor.authorAquino, I. [UNESP]
dc.contributor.authorTsuboy, M. S F [UNESP]
dc.contributor.authorMarcarini, J. C. [UNESP]
dc.contributor.authorMantovani, M. S.
dc.contributor.authorPerazzo, F. F.
dc.contributor.authorMaistro, Edson Luis [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2014-05-27T11:30:02Z
dc.date.available2014-05-27T11:30:02Z
dc.date.issued2013-07-24
dc.description.abstractThe malaria treatment recommended by the World Health Organization involves medicines derived from artemisinin, an active compound extracted from the plant Artemisia annua, and some of its derivatives, such as artesunate. Considering the lack of data regarding the genotoxic effects of these compounds in human cells, the objective of this study was to evaluate the cytotoxicity and genotoxicity, and expressions of the CASP3 and SOD1 genes in a cultured human hepatocellular liver carcinoma cell line (HepG2 cells) treated with artemisinin and artesunate. We tested concentrations of 2.5, 5, 7.5, 10, and 20 μg/mL of both substances with a resazurin cytotoxicity assay, and the concentrations used in the genotoxicity experiments (2.5, 5, and 10 μg/mL) and gene expression analysis (5 mg/mL) were determined. The results of the comet assay in cells treated with artemisinin and artesunate showed a significant dosedependent increase (P < 0.001) in the number of cells with DNA damage at all concentrations tested. However, the gene expression analysis revealed no significant change in expression of CASP3 or SOD1. Our data showed that although artemisinin and artesunate exhibited genotoxic effects in cultured HepG2 cells, they did not significantly alter expression of the CASP3 and SOD1 genes at the doses tested. ©FUNPEC-RP.en
dc.description.affiliationInstituto de Biociências Universidade Estadual Paulista, Botucatu, SP
dc.description.affiliationInstituto de Biociências Universidade Estadual Paulista, Rio Claro, SP
dc.description.affiliationLaboratório de Genética Toxicológica Universidade Estadual de Londrina, Londrina, PR
dc.description.affiliationDepartamento de Ciências Exatas e da Terra Universidade Federal de São Paulo, Diadema, SP
dc.description.affiliationDepartamento de Fonoaudiologia Universidade Estadual Paulista, Marília, SP
dc.description.affiliationUnespInstituto de Biociências Universidade Estadual Paulista, Botucatu, SP
dc.description.affiliationUnespInstituto de Biociências Universidade Estadual Paulista, Rio Claro, SP
dc.description.affiliationUnespDepartamento de Fonoaudiologia Universidade Estadual Paulista, Marília, SP
dc.format.extent2517-2527
dc.identifierhttp://dx.doi.org/10.4238/2013.July.24.6
dc.identifier.citationGenetics and Molecular Research, v. 12, n. 3, p. 2517-2527, 2013.
dc.identifier.doi10.4238/2013.July.24.6
dc.identifier.file2-s2.0-84880814650.pdf
dc.identifier.issn1676-5680
dc.identifier.lattes4787521613038315
dc.identifier.orcid0000-0003-0757-7876
dc.identifier.scopus2-s2.0-84880814650
dc.identifier.urihttp://hdl.handle.net/11449/76047
dc.identifier.wosWOS:000331717400035
dc.language.isoeng
dc.relation.ispartofGenetics and Molecular Research
dc.relation.ispartofsjr0,439
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectArtemisinin
dc.subjectArtesunate
dc.subjectCASP3 and SOD1 genes
dc.subjectComet assay
dc.subjectGene expression
dc.subjectHepG2 cells
dc.subjectartemisinin
dc.subjectartesunate
dc.subjectcaspase 3
dc.subjectcell DNA
dc.subjectdoxorubicin
dc.subjectextracellular superoxide dismutase
dc.subjectresazurin
dc.subjectCasp3 gene
dc.subjectcell count
dc.subjectcell strain HepG2
dc.subjectcomet assay
dc.subjectconcentration response
dc.subjectcontrolled study
dc.subjectcytotoxicity
dc.subjectDNA damage
dc.subjectdrug effect
dc.subjectdrug screening
dc.subjectgene expression
dc.subjectgenotoxicity
dc.subjecthuman
dc.subjecthuman cell
dc.subjectSOD1 gene
dc.titleGenotoxic evaluation of the antimalarial drugs artemisinin and artesunate in human HepG2 cells and effects on CASP3 and SOD1 gene expressionsen
dc.typeArtigo
dcterms.licensehttp://geneticsmr.com/node/2
unesp.author.lattes4787521613038315[6]
unesp.author.orcid0000-0003-0757-7876[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Filosofia e Ciências, Maríliapt

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