Description of three new species of Hepatozoon (Apicomplexa, Hepatozoidae) from Rattlesnakes (Crotalus durissus terrificus) based on molecular, morphometric and morphologic characters

Página do item simplificado
dc.contributor.authorO'Dwyer, Lucia Helena [UNESP]
dc.contributor.authorMoço, Tatiana Cristina [UNESP]
dc.contributor.authorPaduan, Karina dos Santos [UNESP]
dc.contributor.authorSpenassatto, Carine [UNESP]
dc.contributor.authorda Silva, Reinaldo José [UNESP]
dc.contributor.authorRibolla, Paulo Eduardo Martins [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:30:46Z
dc.date.available2014-05-27T11:30:46Z
dc.date.issued2013-10-01
dc.description.abstractHepatozoon spp. are commonly found infecting snakes. Since the latter are parasitized by diverse forms and data in the literature show divergence, we studied Hepatozoon spp. diversity on Crotalus durissus terrificus snakes using both molecular and morphological approaches. Naturally infected animals were employed. Blood was collected, blood smears were prepared and an aliquot was stored at -20. °C for DNA extraction. Five specimens of C. durissus terrificus were selected, each of them infected with one gamont type. Morphological and morphometric analyses of the found gamonts led to their grouping into three populations. For molecular characterization, seven oligonucleotide pairs that amplify distinct regions of rDNA gene were tested by adopting the PCR technique. Only the oligonucleotide pairs HepF300/Hep900 and HEMO1/HEMO2 were efficient in amplifying and distinguishing different isolates of Hepatozoon spp. from snakes. The better results were obtained when both oligonucleotide pairs were used in association. Based on the molecular and morphologic differences, three new species were proposed: Hepatozoon cuestensis sp. nov.; Hepatozoon cevapii sp. nov. and Hepatozoon massardii sp. nov. This is the first description of new Hepatozoon species from snakes, based on molecular characterization and morphological data, in South America. © 2013 Elsevier Inc.en
dc.description.affiliationInstituto de Biociências de Botucatu Unesp - Univ Estadual Paulista, Campus de Botucatu Departamento de Parasitologia, Distrito de Rubião Júnior, Botucatu, SP 18618-970
dc.description.affiliationCEVAP - Center for the Study of Venoms and Venomous Animals - UNESP, Botucatu Campus, SP
dc.description.affiliationUnespInstituto de Biociências de Botucatu Unesp - Univ Estadual Paulista, Campus de Botucatu Departamento de Parasitologia, Distrito de Rubião Júnior, Botucatu, SP 18618-970
dc.description.affiliationUnespCEVAP - Center for the Study of Venoms and Venomous Animals - UNESP, Botucatu Campus, SP
dc.format.extent200-207
dc.identifierhttp://dx.doi.org/10.1016/j.exppara.2013.06.019
dc.identifier.citationExperimental Parasitology, v. 135, n. 2, p. 200-207, 2013.
dc.identifier.doi10.1016/j.exppara.2013.06.019
dc.identifier.issn0014-4894
dc.identifier.issn1090-2449
dc.identifier.lattes3577149748456880
dc.identifier.orcid0000-0001-8735-6090
dc.identifier.scopus2-s2.0-84882247374
dc.identifier.urihttp://hdl.handle.net/11449/76705
dc.identifier.wosWOS:000326058100003
dc.language.isoeng
dc.relation.ispartofExperimental Parasitology
dc.relation.ispartofjcr1.821
dc.relation.ispartofsjr0,635
dc.relation.ispartofsjr0,635
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectHepatozoonen
dc.subjectMolecular characterizationen
dc.subjectMorphologyen
dc.subjectRattlesnakesen
dc.subjectoligonucleotideen
dc.subjectribosome DNAen
dc.subjectApicomplexaen
dc.subjectblood smearen
dc.subjectCrotalusen
dc.subjectDNA extractionen
dc.subjectfemaleen
dc.subjectgamonten
dc.subjectgene amplificationen
dc.subjectHepatozoidaeen
dc.subjecthepatozoonen
dc.subjectHepatozoon cevapiien
dc.subjectHepatozoon cuestensisen
dc.subjectHepatozoon massardiien
dc.subjecthepatozoonosisen
dc.subjectmaleen
dc.subjectmicromorphologyen
dc.subjectmorphometricsen
dc.subjectnew speciesen
dc.subjectnonhumanen
dc.subjectnucleotide sequenceen
dc.subjectpolymerase chain reactionen
dc.subjectpriority journalen
dc.subjectSouth Americaen
dc.subjectspecies differenceen
dc.subjectspecies diversityen
dc.titleDescription of three new species of Hepatozoon (Apicomplexa, Hepatozoidae) from Rattlesnakes (Crotalus durissus terrificus) based on molecular, morphometric and morphologic charactersen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.lattes3577149748456880[6]
unesp.author.orcid0000-0002-3426-6873[5]
unesp.author.orcid0000-0001-8735-6090[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

Arquivos

Coleções

Artigos - Parasitologia - IBB
Botucatu - CEVAP - Centro de Estudos de Venenos e Animais Peçonhentos