Cytotoxic effects of different concentrations of chlorhexidine

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dc.contributor.authorSouza, Lorena Brito de [UNESP]
dc.contributor.authorAquino, Sabrina García de [UNESP]
dc.contributor.authorSouza, Pedro Paulo Chaves de [UNESP]
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorCosta, Carlos Alberto de Souza [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:22:45Z
dc.date.available2014-05-27T11:22:45Z
dc.date.issued2007-12-01
dc.description.abstractPurpose: To evaluate the cytotoxic effects of different concentrations of Chlorhexidine (Chx) to the odontoblast cell line MDPC-23. Methods: The odontoblast-like cells were seeded (30,000 cells/cm 2) in 60 wells of 24-well dishes and then incubated in contact with the following experimental and control solutions: Group 1: 0.0024% Chx; Group 2: 0.004% Chx; Group 3: 0.02% Chx; Group 4: Phosphate buffer saline solution (PBS, negative control); and Group 5: 0.06% H 2O 2 (positive control). Cell metabolic activity was measured by MTT assay and the cell morphology was analyzed by SEM. Results: The cytotoxic effects of Chx are dose-dependent. The reduction in the cell metabolism for Groups 1, 2, and 3 was 24.8%, 29.9% and 70.8%, respectively. No statistical difference was observed between the Groups 1 and 2 in which no significant cell morphology changes occurred. Consequently, it was concluded that 0.02% Chx solution presents high cytotoxicity to the odontoblast-like cells MDPC-23. On theother hand, 0.0024% and 0.004% Chx causes slight cytopathic effects to the cultured cells.en
dc.description.affiliationUniversity of São Paulo State UNESP, Rua Humaitá 1680, CEP: 14.801-903, CP: 331-Centro, Araraquara, SP
dc.description.affiliationDepartment of Physiology and Pathology School of Dentistry University of São Paulo State, Araraquara, SP
dc.description.affiliationDepartment of Biochemistry Medical School University of São Paulo, USP, Ribeirão Preto, SP
dc.description.affiliationDepartment of Orthodontics and Pediatric Dentistry School of Dentistry
dc.description.affiliationDepartment of Physiology and Pathology University of São Paulo State UNESP, Araraquara, SP
dc.description.affiliationUnespUniversity of São Paulo State UNESP, Rua Humaitá 1680, CEP: 14.801-903, CP: 331-Centro, Araraquara, SP
dc.description.affiliationUnespDepartment of Physiology and Pathology University of São Paulo State UNESP, Araraquara, SP
dc.format.extent400-404
dc.identifierhttp://www.amjdent.com/previousarticles.htm
dc.identifier.citationAmerican Journal of Dentistry, v. 20, n. 6, p. 400-404, 2007.
dc.identifier.issn0894-8275
dc.identifier.lattes4517484241515548
dc.identifier.scopus2-s2.0-38349140458
dc.identifier.urihttp://hdl.handle.net/11449/70206
dc.identifier.wosWOS:000251825800011
dc.language.isoeng
dc.relation.ispartofAmerican Journal of Dentistry
dc.relation.ispartofjcr0.760
dc.relation.ispartofsjr0,509
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectbuffer
dc.subjectchlorhexidine
dc.subjectcoloring agent
dc.subjectdiagnostic agent
dc.subjecthydrogen peroxide
dc.subjectoxidizing agent
dc.subjectsodium chloride
dc.subjecttetrazolium
dc.subjectthiazole derivative
dc.subjectthiazolyl blue
dc.subjecttopical antiinfective agent
dc.subjectunclassified drug
dc.subjectanimal
dc.subjectcell adhesion
dc.subjectcell line
dc.subjectcell membrane
dc.subjectcell shape
dc.subjectcomparative study
dc.subjectdose response
dc.subjectdrug effect
dc.subjectmetabolism
dc.subjectmouse
dc.subjectodontoblast
dc.subjectpathology
dc.subjectultrastructure
dc.subjectAnimals
dc.subjectAnti-Infective Agents, Local
dc.subjectBuffers
dc.subjectCell Adhesion
dc.subjectCell Line
dc.subjectCell Membrane
dc.subjectCell Shape
dc.subjectChlorhexidine
dc.subjectColoring Agents
dc.subjectDose-Response Relationship, Drug
dc.subjectHydrogen Peroxide
dc.subjectMice
dc.subjectOdontoblasts
dc.subjectOxidants
dc.subjectSodium Chloride
dc.subjectTetrazolium Salts
dc.subjectThiazoles
dc.titleCytotoxic effects of different concentrations of chlorhexidineen
dc.typeArtigo
unesp.author.lattes4517484241515548[5]
unesp.author.orcid0000-0002-7455-6867[5]
unesp.author.orcid0000-0001-7266-6061[3]
unesp.author.orcid0000-0002-2462-6759[1]
unesp.author.orcid0000-0002-3988-1939[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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