Cell cycle kinetics, apoptosis rates and gene expressions of MDR-1, TP53, BCL-2 and BAX in transmissible venereal tumour cells and their association with therapy response

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dc.contributor.authorFlórez, M. M. [UNESP]
dc.contributor.authorFêo, H. B. [UNESP]
dc.contributor.authorda Silva, G. N.
dc.contributor.authorYamatogi, R. S. [UNESP]
dc.contributor.authorAguiar, A. J. [UNESP]
dc.contributor.authorAraújo, J. P. [UNESP]
dc.contributor.authorRocha, N. S. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidad de Caldas
dc.contributor.institutionUniversidade Federal de Ouro Preto
dc.date.accessioned2018-12-11T17:01:14Z
dc.date.available2018-12-11T17:01:14Z
dc.date.issued2017-09-01
dc.description.abstractTransmissible venereal tumour (TVT) generally presents different degrees of aggressiveness, which makes them unresponsive to conventional treatment protocols. This implies a progressive alteration of their biological profile. This study aimed to evaluate the cytotoxicity, cell survival, apoptosis and cell cycle alterations in TVT cell cultures subjected to treatment with vincristine. Similarly, it assessed possible implications of MDR-1, TP53, BCL-2, and BAX gene expressions in eight TVT primary cultures for both resistance to chemotherapy and biological behaviour. When comparing TVT cells receiving vincristine to those untreated, a statistical difference related to increased cytotoxicity and decreased survival rates, and alterations in G1 and S cell cycle phases were found but without detectable differences in apoptosis. Increased MDR-1 gene expression was observed after treatment. The groups did not differ statistically in relation to the TP53, BAX and BCL-2 genes. Although preliminary, the findings suggest that such augmented expression is related to tumour malignancy and chemotherapy resistance.en
dc.description.affiliationDepartment of Veterinary Clinics Faculty of Veterinary Medicine São Paulo State University—UNESP
dc.description.affiliationVeterinary Pathology Research Group Faculty of Agricultural Sciences Universidad de Caldas
dc.description.affiliationDepartment of Clinical Analysis. Pharmacy School Universidade Federal de Ouro Preto
dc.description.affiliationDepartment of Microbiology and immunology Institute of Biosciences of Botucatu (IBB) and Biotechnology Institute (IBTEC) São Pablo State University—UNESP
dc.description.affiliationUnespDepartment of Veterinary Clinics Faculty of Veterinary Medicine São Paulo State University—UNESP
dc.description.affiliationUnespDepartment of Microbiology and immunology Institute of Biosciences of Botucatu (IBB) and Biotechnology Institute (IBTEC) São Pablo State University—UNESP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent793-807
dc.identifierhttp://dx.doi.org/10.1111/vco.12220
dc.identifier.citationVeterinary and Comparative Oncology, v. 15, n. 3, p. 793-807, 2017.
dc.identifier.doi10.1111/vco.12220
dc.identifier.issn1476-5829
dc.identifier.issn1476-5810
dc.identifier.scopus2-s2.0-84959106167
dc.identifier.urihttp://hdl.handle.net/11449/172586
dc.language.isoeng
dc.relation.ispartofVeterinary and Comparative Oncology
dc.relation.ispartofsjr0,946
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectapoptosis
dc.subjectchemotherapy
dc.subjectcytotoxicity
dc.subjectMDR-1
dc.subjecttoxicogenomic
dc.titleCell cycle kinetics, apoptosis rates and gene expressions of MDR-1, TP53, BCL-2 and BAX in transmissible venereal tumour cells and their association with therapy responseen
dc.typeArtigo
unesp.author.lattes7162819131462797[5]
unesp.author.orcid0000-0001-6854-722X[5]

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Artigos - Microbiologia e Imunologia - IBB