Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization–time of flight mass spectrometry and cydB real-time quantitative PCR

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dc.contributor.authorPizauro, Lucas J.L. [UNESP]
dc.contributor.authorde Almeida, Camila C. [UNESP]
dc.contributor.authorSoltes, Glenn A.
dc.contributor.authorSlavic, Durda
dc.contributor.authorRossi-Junior, Oswaldo D. [UNESP]
dc.contributor.authorde Ávila, Fernando. A. [UNESP]
dc.contributor.authorZafalon, Luiz. F.
dc.contributor.authorMacInnes, Janet I.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Guelph
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.date.accessioned2018-12-11T17:11:13Z
dc.date.available2018-12-11T17:11:13Z
dc.date.issued2017-05-01
dc.description.abstractIncorrect identification of Staphylococcus spp. can have serious clinical and zoonotic repercussions. Accordingly, the aim of this study was to determine if matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) and/or cydB real- time quantitative PCR (qPCR) could be used to accurately identify coagulase negative Staphylococcus spp. (CoNS) obtained from buffalo milk and milking environment samples. Seventy-five of 84 CoNS isolates could be identified to the species level (score value >1.99) using MALDI-TOF MS. However, as determined by cytochrome d ubiquinol oxidase subunit II (cydB) qPCR and by 16S RNA and cydB gene sequencing, 10 S. agnetis strains were wrongly identified as S. hyicus by MALDI-TOF MS. In addition, 9 isolates identified by MALDI-TOF only to the genus level (score values between 1.70 and 1.99) could be identified to species by cydB qPCR. Our findings suggest that MALDI-TOF MS is a reliable method for rapid identification of S. chromogenes and S. epidermidis (species of interest both in human and veterinary medicine) and may be able to correctly identify other Staphylococcus spp. However, at present not all Staphylococcus spp. found in buffalo milk can be accurately identified by MALDI-TOF MS and for these organisms, the cydB qPCR developed in the current study may provide a reliable alternative method for rapid identification of CoNS species.en
dc.description.affiliationDepartment of Veterinary Preventive Medicine and Animal Reproduction São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, Jaboticabal
dc.description.affiliationDepartment of Veterinary Pathology São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, Jaboticabal
dc.description.affiliationUniversity of Guelph Department of Pathobiology, 50 Stone Rd. East
dc.description.affiliationAnimal Health Laboratory University of Guelph, Post Office 3612
dc.description.affiliationBrazilian Agricultural Research Corporation (EMBRAPA) Embrapa Southeast Livestock, Washington Luiz road, Km 234Washington Luiz road, Km 234
dc.description.affiliationUnespDepartment of Veterinary Preventive Medicine and Animal Reproduction São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, Jaboticabal
dc.description.affiliationUnespDepartment of Veterinary Pathology São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, Jaboticabal
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent8-14
dc.identifierhttp://dx.doi.org/10.1016/j.vetmic.2017.03.036
dc.identifier.citationVeterinary Microbiology, v. 204, p. 8-14.
dc.identifier.doi10.1016/j.vetmic.2017.03.036
dc.identifier.file2-s2.0-85017429123.pdf
dc.identifier.issn1873-2542
dc.identifier.issn0378-1135
dc.identifier.scopus2-s2.0-85017429123
dc.identifier.urihttp://hdl.handle.net/11449/174461
dc.language.isoeng
dc.relation.ispartofVeterinary Microbiology
dc.relation.ispartofsjr1,175
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectcydB qPCR
dc.subjectMALDI-TOF MS
dc.subjectMilk
dc.subjectStaphylococcus agnetis
dc.subjectStaphylococcus hyicus
dc.titleSpecies level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization–time of flight mass spectrometry and cydB real-time quantitative PCRen
dc.typeArtigo
unesp.author.lattes0746647601766390[6]
unesp.author.orcid0000-0002-9779-2213[6]

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Artigos - Medicina Veterinária Preventiva e Reprodução Animal - FCAV
Artigos - Patologia Veterinária - FCAV