Tonic modulation of anxiety-like behavior by corticotropin-releasing factor (CRF) type 1 receptor (CRF1) within the medial prefrontal cortex (mPFC) in male mice: Role of protein kinase A (PKA)

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dc.contributor.authorMiguel, Tarciso Tadeu [UNESP]
dc.contributor.authorGames, Karina Santos [UNESP]
dc.contributor.authorNunes-de-Souza, Ricardo Luiz [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-03-18T15:54:10Z
dc.date.available2015-03-18T15:54:10Z
dc.date.issued2014-07-01
dc.description.abstractThe medial prefrontal cortex (mPFC) and the neuropeptide corticotropin-releasing factor (CRF) have recently been receiving more attention from those interested in the neurobiology of anxiety. Here, we investigated the CRF pathway in the modulation of anxiety-like behaviors in male mice exposed to the elevated plus-maze (EPM), through intra-mPFC injections of CRF, CP376395 [N-(1-ethylpropyl)-3,6-dimethyl-2-(2,4,6-trimethylphenoxy)-4-pyridinamine hydrochloride, a CRF type 1 receptor antagonist (CR F1)] or H-89 [N-[2-[[3-(4-bromophenyl)-2-propenyl]amino]ethy11-5 Aso quinolinesulf onamide dihydrochloride, a protein kinase (PICA) inhibitor]. We also investigated the effects of intra-mPFC injections of H-89 on the behavioral effects induced by CRF. Mice received bilateral intra-mPFC injections of CRF (0, 37.5, 75 or 150 pmol), CP376395 (0, 0.75, 1.5 or 3 nmol) or H-89 (0, 1.25, 2.5 or 5 nmol) and were exposed to the EPM, to record conventional and complementary measures of anxiety for 5 mm. Results showed that while CRF (75 and 150 pmol) produced an anxiogenic-like effect, CP376395 (all doses) and H-89 (5 nmol) attenuated anxiety-like behavior. When injected before CRF (150 pmol), intra-mPFC H-89 (2.5 nmol, a dose devoid of intrinsic effects on anxiety) completely blocked the anxiogenic-like effects of CRF. These results suggest that (i) CRF plays a tonic anxiogenic-like role at CRF1 receptors within the mPFC, since their blockade per se attenuated anxiety indices and (ii) the anxiogenic-like effects following CRF1 receptor activation depend on CAMP/PICA cascade activation in this limbic forebrain area. (C) 2014 Elsevier Inc All rights reserved.en
dc.description.affiliationUNESP, Fac Ciencias Farmaceut, Farmacol Lab, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Ciencias Farmaceut, Farmacol Lab, BR-14801902 Araraquara, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipPROPe-UNESP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdCNPq: 478696/2013-2
dc.description.sponsorshipIdCNPq: 305597/2012-4
dc.description.sponsorshipIdFAPESP: 13/01283-6
dc.description.sponsorshipIdFAPESP: 10/01290-4
dc.description.sponsorshipIdPROPe-UNESP1932/009/13
dc.description.sponsorshipIdCAPES: 2748/2010
dc.format.extent247-256
dc.identifierhttp://dx.doi.org/10.1016/j.yhbeh.2014.05.003
dc.identifier.citationHormones And Behavior. San Diego: Academic Press Inc Elsevier Science, v. 66, n. 2, p. 247-256, 2014.
dc.identifier.doi10.1016/j.yhbeh.2014.05.003
dc.identifier.issn0018-506X
dc.identifier.urihttp://hdl.handle.net/11449/116801
dc.identifier.wosWOS:000340212400005
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofHormones And Behavior
dc.relation.ispartofjcr4.418
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAnxietyen
dc.subjectCorticotrophin-releasing factor (Cm)en
dc.subjectCRF1 receptoren
dc.subjectPKAen
dc.subjectMedial prefrontal cortex (mPFC)en
dc.subjectElevated plus mazeen
dc.subjectMiceen
dc.titleTonic modulation of anxiety-like behavior by corticotropin-releasing factor (CRF) type 1 receptor (CRF1) within the medial prefrontal cortex (mPFC) in male mice: Role of protein kinase A (PKA)en
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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