The role of the TP53 gene during rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide

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dc.contributor.authorMinicucci, E. M. [UNESP]
dc.contributor.authorRibeiro, D. A.
dc.contributor.authorda Silva, G. N. [UNESP]
dc.contributor.authorPardini, Maria Ines de Moura Campos [UNESP]
dc.contributor.authorMontovani, Jair Cortez [UNESP]
dc.contributor.authorSalvadori, Daisy Maria Favero [UNESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:37:36Z
dc.date.available2014-05-20T13:37:36Z
dc.date.issued2011-07-01
dc.description.abstractThe medium-term tongue carcinogenesis assay is a useful model for studying oral squamous cell carcinomas phase by phase. The aim of the present study was to investigate the expression of p53 by immunohistochemistry and examine the DNA sequence of exons 5, 6, 7, and 8 of Tp53 for mutations during rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide (4NQO). A total of 30 male Wistar rats were treated with 4-nitroquinoline 1-oxide in their drinking water for 4, 12, and 20 weeks at a dose of 50 ppm. Ten animals were used as negative controls. No histopathological changes in the tongue epithelia were observed in the control group or in the treatment group after 4 weeks of 4NQO. Following 12 weeks of treatment, hyperplasia as well as epithelial dysplasia was found in both mild and moderate forms. At 20 weeks, moderate and/or severe oral dysplasia and squamous cell carcinoma of the tongue were found, and the majority of animals had squamous cell carcinoma. The levels of p53 protein were increased (p < 0.05) in pre-neoplastic lesions and in squamous cell carcinomas in some of the tumor cells in squamous cell carcinomas. No mutations were found in any of the exons that were evaluated after the 4-, 12-, or 20-week treatments. Taken together, our results suggest that p53 expression may be an important event in the malignant conversion, whereas Tp53 mutations are not involved in the multi-step tongue carcinogenesis of Wistar rats induced by 4NQO. (C) 2010 Elsevier GmbH. All rights reserved.en
dc.description.affiliationUniv Fed São Paulo, UNIFESP, Dept Biosci, BR-11060001 Santos, SP, Brazil
dc.description.affiliationSão Paulo State Univ, UNESP, Dept Dermatol & Radiotherapy, São Paulo, SP, Brazil
dc.description.affiliationSão Paulo State Univ, UNESP, Dept Pathol, São Paulo, SP, Brazil
dc.description.affiliationSão Paulo State Univ, UNESP, Dept Clin Med, São Paulo, SP, Brazil
dc.description.affiliationSão Paulo State Univ, UNESP, Dept Ophthalmol & Otorhinolaryngol, São Paulo, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Dept Dermatol & Radiotherapy, São Paulo, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Dept Pathol, São Paulo, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Dept Clin Med, São Paulo, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Dept Ophthalmol & Otorhinolaryngol, São Paulo, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 05/59156-3
dc.description.sponsorshipIdFAPESP: 07/01228-4
dc.description.sponsorshipIdCNPq: 561357/2008-0
dc.format.extent483-489
dc.identifierhttp://dx.doi.org/10.1016/j.etp.2010.03.009
dc.identifier.citationExperimental and Toxicologic Pathology. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 63, n. 5, p. 483-489, 2011.
dc.identifier.doi10.1016/j.etp.2010.03.009
dc.identifier.issn0940-2993
dc.identifier.lattes6121068386940628
dc.identifier.lattes5051118752980903
dc.identifier.lattes4619588334582084
dc.identifier.urihttp://hdl.handle.net/11449/13035
dc.identifier.wosWOS:000291706600011
dc.language.isoeng
dc.publisherElsevier Gmbh, Urban & Fischer Verlag
dc.relation.ispartofExperimental and Toxicologic Pathology
dc.relation.ispartofjcr2.023
dc.relation.ispartofsjr0,551
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectTp53en
dc.subjectRat tongue mucosaen
dc.subjectOral squamous cell carcinomaen
dc.subject4-Nitroquinoline 1-oxideen
dc.subjectDirect DNA-sequencingen
dc.titleThe role of the TP53 gene during rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxideen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier Gmbh, Urban & Fischer Verlag
unesp.author.lattes6121068386940628
unesp.author.lattes5051118752980903
unesp.author.lattes4619588334582084
unesp.author.orcid0000-0001-9323-3134[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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