Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells

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dc.contributor.authorOcampo, Pablo E. [UNESP]
dc.contributor.authorVallejo, Viviana [UNESP]
dc.contributor.authorMontoya, Luis M.
dc.contributor.authorRocha, Noeme S. [UNESP]
dc.contributor.authorLandim, Fernanda da C. [UNESP]
dc.contributor.authorRahal, Sheila C. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionResearch Group in Veterinary Pathology
dc.date.accessioned2022-05-01T06:02:33Z
dc.date.available2022-05-01T06:02:33Z
dc.date.issued2021-07-01
dc.description.abstractBackground: Osteoarthritis is a complex degenerative disease with several factors contributing to joint damage. Objective: To compare the potential effect of hyaluronic acid (HA) and triamcinolone acetonide (TA), alone or combined, on the in vitro chondrogenic differentiation process of mesenchymal stem cells (MSCs). Methods: MSCs were divided into four groups: Control, HA, TA, and HA/TA combined. Each treatment group was cultured for 14 days in chondrogenic differentiation medium. The chondrogenic differentiation potential was assessed by histology and immunohistochemistry. Results: The HA and HA/TA-treated MSCs presented histological characteristics similar to native chondrocytes. The extracellular matrix (ECM) of TA-treated MSCs was compact and organized. Glycosaminoglycan staining was intense in Control, moderate in TA, slight in HA/TA, and undetectable in HA. Type II collagen immunoreactivity was high in the TA-treated ECM and MSCs. Conclusions: Histological analysis shows that HA influences morphological development similar to chondrocytes of the MSCs, but with low expression of specific cartilage molecules. The TA promotes formation of a compact and organized ECM.en
dc.description.affiliationSão Paulo State University (Unesp) School of Veterinary Medicine and Animal Science
dc.description.affiliationFaculty of Veterinary Medicine Universidad Nacional de Colombia Research Group in Veterinary Pathology
dc.description.affiliationUnespSão Paulo State University (Unesp) School of Veterinary Medicine and Animal Science
dc.format.extent212-223
dc.identifierhttp://dx.doi.org/10.17533/udea.rccp.v34n3a06
dc.identifier.citationRevista Colombiana de Ciencias Pecuarias, v. 34, n. 3, p. 212-223, 2021.
dc.identifier.doi10.17533/udea.rccp.v34n3a06
dc.identifier.issn2256-2958
dc.identifier.issn0120-0690
dc.identifier.scopus2-s2.0-85110174025
dc.identifier.urihttp://hdl.handle.net/11449/233265
dc.language.isoeng
dc.relation.ispartofRevista Colombiana de Ciencias Pecuarias
dc.sourceScopus
dc.subjectCartilage
dc.subjectCell differentiation
dc.subjectChondrocytes
dc.subjectChondrogenesis
dc.subjectChondrogenic differentiation
dc.subjectCollagen
dc.subjectCorticosteroids
dc.subjectGlucocorticoid
dc.subjectHistology
dc.subjectHyaluronic acid
dc.subjectMesenchymal stem cell
dc.subjectOsteoarthritis
dc.subjectTriancinolone acetonide
dc.subjectViscosupplementation
dc.titlePotential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cellsen
dc.titleEfecto potencial del ácido hialurónico y del acetato de triamcinolona solos o combinados sobre la diferenciación condrogénica de células-tronco mesenquimaleses
dc.titleEfeito potencial do ácido hialurônico e triancinolona acetonida, isolados e combinados, na diferenciação condrogênica de células-tronco mensenquimaispt
dc.typeArtigo
unesp.author.orcid0000-0001-8504-5926[1]
unesp.author.orcid0000-0002-6270-261X[2]
unesp.author.orcid0000-0002-5198-7998[3]
unesp.author.orcid0000-0002-8188-8149[4]
unesp.author.orcid0000-0002-2420-2550[5]

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