Biotin self-assembled monolayer for impedimetric genosensor for direct detection of HIV-1

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dc.contributor.authorTamayo, Abel I. Balbin
dc.contributor.authorRizo, Laura S. López
dc.contributor.authorde Armas, Madelin Blanco
dc.contributor.authorFerreira, Antonio Aparecido Pupim [UNESP]
dc.contributor.authorManzani, Danilo
dc.contributor.authorYamanaka, Hideko [UNESP]
dc.contributor.authorGuas, Ana Margarita Esteva
dc.contributor.institutionUniversity of Havana
dc.contributor.institutionAIDS Research Laboratory (LISIDA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2020-12-12T02:30:48Z
dc.date.available2020-12-12T02:30:48Z
dc.date.issued2020-03-01
dc.description.abstractThis study was performed to prove the feasibility of a direct impedimetric DNA genosensor by using biotin self-assembled monolayer (SAM). The sensor was composed of a gold rod with a SAM of biotin-modified 19-base oligonucleotide probe (5´-AAA GAT TAA TCC TGG G -3´,) for HIV-1. The electrode modification was characterized by Surface-Enhancement Raman Spectroscopy (SERS), cyclic voltammetry and the hybridization with the complementary oligonucleotide was monitored by impedimetric technique. SERS was used to confirm the linking of Biotin-DNA on the surface of the Au electrode. The coverage was 89% of the surface and a concentration of complementary oligonucleotides of 2.5 10− 12 molL−1 could be detected.en
dc.description.affiliationDepartment of Analytical Chemistry University of Havana
dc.description.affiliationAIDS Research Laboratory (LISIDA)
dc.description.affiliationDepartment of Analytical Chemistry São Paulo State University (UNESP)
dc.description.affiliationSão Carlos Institute of Chemistry Department of Chemistry and Molecular Physics University of São Paulo (USP)
dc.description.affiliationUnespDepartment of Analytical Chemistry São Paulo State University (UNESP)
dc.identifierhttp://dx.doi.org/10.1016/j.microc.2019.104462
dc.identifier.citationMicrochemical Journal, v. 153.
dc.identifier.doi10.1016/j.microc.2019.104462
dc.identifier.issn0026-265X
dc.identifier.scopus2-s2.0-85075892190
dc.identifier.urihttp://hdl.handle.net/11449/201371
dc.language.isoeng
dc.relation.ispartofMicrochemical Journal
dc.sourceScopus
dc.subjectDNA biosensors
dc.subjectGenosensor
dc.subjectHIV-1
dc.subjectHybridization
dc.subjectImpedimetric
dc.subjectProviral DNA
dc.titleBiotin self-assembled monolayer for impedimetric genosensor for direct detection of HIV-1en
dc.typeArtigo

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