Further characterization of the subunits of the giant extracellular hemoglobin of Glossoscolex paulistus (HbGp) by SDS-PAGE electrophoresis and MALDI-TOF-MS
dc.contributor.author | Carvalho, Francisco Adriano O. | |
dc.contributor.author | Carvalho, José Wilson P. | |
dc.contributor.author | Santiago, Patrícia S. [UNESP] | |
dc.contributor.author | Tabak, Marcel | |
dc.contributor.institution | Universidade de São Paulo (USP) | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2014-05-27T11:26:06Z | |
dc.date.available | 2014-05-27T11:26:06Z | |
dc.date.issued | 2011-11-01 | |
dc.description.abstract | Further characterization of hemoglobin of Glossoscolex paulistus (HbGp) subunits was performed based on SDS-PAGE, size exclusion chromatography (SEC) and MALDI-TOF-MS analysis. SDS-PAGE has shown a total of four linker chains, two quite intense and two of lower intensity. HbGp fractions (I-VI), obtained by size exclusion chromatography (SEC), from oligomeric dissociation at alkaline pH 9.6, were monitored. Fraction I is identical to the whole protein. The monomeric chains c, obtained from the trimer abc reduction, present four isoforms with MM 17,336 Da, 17,414 Da, 17,546 Da and 17,620 Da. Furthermore, the trimer subunit presents two isoforms, T 1 and T 2, with MM 51,200 ± 60 and 51,985 ± 50 Da, respectively. Based on SDS-PAGE, the linker chains seem to be distributed along the different fractions of the SEC chromatogram, appearing along the peaks corresponding to fractions I-V. The fraction IV contains, predominantly, trimers with some linkers contamination. The strong interaction of linker chains L with the trimers abc, makes it difficult to obtain these subunits in pure form. The monomer d in fraction VI appears to be quite pure, in agreement with previous studies. © 2011 Elsevier Ltd. All rights reserved. | en |
dc.description.affiliation | Instituto de Química de São Carlos Universidade de São Paulo, São Carlos, SP | |
dc.description.affiliation | Universidade Estadual Paulista Júlio de Mesquita Filho Campus Experimental de Registro, SP | |
dc.description.affiliationUnesp | Universidade Estadual Paulista Júlio de Mesquita Filho Campus Experimental de Registro, SP | |
dc.format.extent | 2144-2151 | |
dc.identifier | http://dx.doi.org/10.1016/j.procbio.2011.08.013 | |
dc.identifier.citation | Process Biochemistry, v. 46, n. 11, p. 2144-2151, 2011. | |
dc.identifier.doi | 10.1016/j.procbio.2011.08.013 | |
dc.identifier.file | 2-s2.0-80053956145.pdf | |
dc.identifier.issn | 1359-5113 | |
dc.identifier.lattes | 6705367010662087 | |
dc.identifier.orcid | 0000-0002-6205-9441 | |
dc.identifier.scopus | 2-s2.0-80053956145 | |
dc.identifier.uri | http://hdl.handle.net/11449/72767 | |
dc.language.iso | eng | |
dc.relation.ispartof | Process Biochemistry | |
dc.relation.ispartofjcr | 2.616 | |
dc.relation.ispartofsjr | 0,761 | |
dc.rights.accessRights | Acesso aberto | |
dc.source | Scopus | |
dc.subject | Electrophoresis | en |
dc.subject | Extracellular hemoglobin | en |
dc.subject | Glossosocolex paulistus | en |
dc.subject | MALDI-TOF-MS | en |
dc.subject | Subunits characterization | en |
dc.subject | Subunits molecular masses | en |
dc.subject | Extracellular | en |
dc.subject | MALDI TOF MS | en |
dc.subject | Alkalinity | en |
dc.subject | Characterization | en |
dc.subject | Chromatographic analysis | en |
dc.subject | Gel permeation chromatography | en |
dc.subject | Hemoglobin | en |
dc.subject | Inductively coupled plasma | en |
dc.subject | Oligomers | en |
dc.subject | Size exclusion chromatography | en |
dc.subject | Glossoscolex | en |
dc.title | Further characterization of the subunits of the giant extracellular hemoglobin of Glossoscolex paulistus (HbGp) by SDS-PAGE electrophoresis and MALDI-TOF-MS | en |
dc.type | Artigo | |
dcterms.license | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
unesp.author.lattes | 6705367010662087[3] | |
unesp.author.orcid | 0000-0002-6205-9441[3] | |
unesp.campus | Universidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias do Vale do Ribeira, Registro | pt |
unesp.department | Engenharia Agronômica - FCAVR | pt |
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