Effects of gaseous atmosphere and antioxidants on the development and cryotolerance of bovine embryos at different periods of in vitro culture

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dc.contributor.authorSouza Rocha-Frigoni, Nathalia Alves de [UNESP]
dc.contributor.authorSilva Leao, Beatriz Caetano da [UNESP]
dc.contributor.authorNogueira, Eriklis
dc.contributor.authorAccorsi, Monica Ferreira [UNESP]
dc.contributor.authorMingoti, Gisele Zoccal [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.date.accessioned2015-10-21T13:10:07Z
dc.date.available2015-10-21T13:10:07Z
dc.date.issued2015-04-01
dc.description.abstractThis study examined the effects of antioxidant supplementation and O-2 tension on embryo development, cryotolerance and intracellular reactive oxygen species (ROS) levels. The antioxidant supplementation consisted of 0.6 mM cysteine (CYST); 0.6 mM cysteine + 100 mu M cysteamine (C+C); 100 IU catalase (CAT) or 100 mu M beta-mercaptoethanol (beta-ME) for 3 or 7 days of in vitro culture (IVC). Two O-2 tensions (20% O-2 [5% CO2 in air] or 7% O-2, 5% CO2 and 88% N-2 [gaseous mixture]) were examined. After 7 days of antioxidant supplementation, the blastocyst frequencies were adversely affected (P<0.05) by CYST (11.2%) and C+C (1.44%), as well as by low O-2 tension (17.2% and 11.11% for 20% and 7% O-2, respectively) compared with the control (26.6%). The blastocyst re-expansion rates were not affected (P > 0.05) by the treatments (range, 66-100%). After 3 days of antioxidant supplementation, the blastocyst frequencies were not affected (P > 0.05) by any of the antioxidants (range, 43.6-48.5%), but they were reduced by low O-2 tension (P<0.05) (52.1% and 38.4% for 20% and 7% O-2, respectively). The intracellular ROS levels, demonstrated as arbitrary fluorescence units, were not affected (P > 0.05) by antioxidant treatment (range, 0.78 to 0.95) or by O-2 tension (0.86 and 0.88 for 20% and 7% O-2, respectively). The re-expansion rates were not affected (P > 0.05) by any of the treatments (range, 63.6-93.3%). In conclusion, intracellular antioxidant supplementation and low O-2 tension throughout the entire IVC period were deleterious to embryo development. However, antioxidant supplementation up to day 3 of IVC did not affect the blastocyst frequencies or intracellular ROS levels.en
dc.description.affiliationSchool of Veterinary Medicine, Department of Animal Health, UNESP–Universidade Estadual Paulista, Araçatuba 16050–680, São Paulo, Brazil
dc.description.affiliationSchool of Veterinary Medicine, Laboratory of Reproductive Physiology, UNESP–Universidade Estadual Paulista, Araçatuba, SP 16050–680, Brazil.
dc.description.affiliationBrazilian Agricultural Research Corporation, EMBRAPA Pantanal, Corumbá, MS 79320–900, Brazil.
dc.description.affiliationUnespSchool of Veterinary Medicine, Department of Animal Health, UNESP–Universidade Estadual Paulista, Araçatuba 16050–680, São Paulo, Brazil
dc.description.affiliationUnespSchool of Veterinary Medicine, Laboratory of Reproductive Physiology, UNESP–Universidade Estadual Paulista, Araçatuba, SP 16050–680, Brazil.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 2011/18257-2
dc.format.extent159-168
dc.identifier.citationZygote. New York: Cambridge Univ Press, v. 23, n. 2, p. 159-168, 2015.
dc.identifier.doi10.1017/S0967199413000361
dc.identifier.issn0967-1994
dc.identifier.orcid0000-0002-3059-4458
dc.identifier.urihttp://hdl.handle.net/11449/128467
dc.identifier.wosWOS:000352708100001
dc.language.isoeng
dc.publisherCambridge Univ Press
dc.relation.ispartofZygote
dc.relation.ispartofjcr1.114
dc.relation.ispartofsjr0,387
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAntioxidantsen
dc.subjectCryotoleranceen
dc.subjectEmbryo cultureen
dc.subjectEmbryo developmenten
dc.subjectGaseous atmosphereen
dc.titleEffects of gaseous atmosphere and antioxidants on the development and cryotolerance of bovine embryos at different periods of in vitro cultureen
dc.typeArtigo
dcterms.licensehttp://journals.cambridge.org/action/displaySpecialPage?pageId=4676
dcterms.rightsHolderCambridge Univ Press
unesp.author.orcid0000-0002-3059-4458[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araçatubapt

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