Detection of Leishmania (L.) infantum in stray dogs by molecular techniques with sensitive species-specific primers

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dc.contributor.authorSilva, Rodrigo C.
dc.contributor.authorRichini-Pereira, Virgínia B.
dc.contributor.authorKikuti, Mariana
dc.contributor.authorMarson, Pâmela M. [UNESP]
dc.contributor.authorLangoni, Helio [UNESP]
dc.contributor.institutionMississippi State
dc.contributor.institutionAdolfo Lutz Institute
dc.contributor.institutionUniversidade Federal da Bahia (UFBA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:07:44Z
dc.date.available2018-12-11T17:07:44Z
dc.date.issued2017-11-01
dc.description.abstractBackground: Canine visceral leishmaniasis (CVL) is a worldwide parasitic zoonosis caused by Leishmania (Leishmania) infantum around the world. Canids are the definitive hosts and sand flies the intermediate hosts. Objective: To test the hypothesis that a new species-specific primers (Lch14:Lch15, targeting a multiple alignment for L. infantum kDNA minicircle) is an efficient diagnostic tool for L. infantum. Methods: The presence of L. infantum DNA was assessed in blood samples of 69 stray dogs using the conventional PCR (cPCR) and quantitative PCR (qPCR). Additional 50 lymph nodes and 50 bone marrow samples (positive and negative samples for parasitological tests) from dogs from endemic and nonendemic areas for CVL were also used. Results: L. infantum strains, and all positive lymph node and bone marrow samples for parasitological test gave positive results for cPCR and qPCR, presenting analytical sensitivity of ∼10° parasite mL−1. For the blood samples, 40/69 (58%; CI 95%; 46%-69%) resulted positive for L. infantum in both tests. All positive samples were confirmed by sequencing. Conclusion: This study showed the importance of the specific detection of L. infantum based on species-specific primers by molecular techniques, highlighting the application as a confirmation method in epidemiological studies and to adopt the best control measures.en
dc.description.affiliationDepartment of Pathobiology and Population Medicine College of Veterinary Medicine Mississippi State University Mississippi State
dc.description.affiliationRegional Laboratory of Bauru Adolfo Lutz Institute
dc.description.affiliationInstitute of Collective Health Federal University of Bahia
dc.description.affiliationDepartment of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Science UNESP Universidade Estadual Paulista
dc.description.affiliationUnespDepartment of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Science UNESP Universidade Estadual Paulista
dc.description.sponsorshipFundação Oswaldo Cruz
dc.format.extent23-30
dc.identifierhttp://dx.doi.org/10.1080/01652176.2016.1252073
dc.identifier.citationVeterinary Quarterly, v. 37, n. 1, p. 23-30, 2017.
dc.identifier.doi10.1080/01652176.2016.1252073
dc.identifier.file2-s2.0-84994827752.pdf
dc.identifier.issn1875-5941
dc.identifier.issn0165-2176
dc.identifier.scopus2-s2.0-84994827752
dc.identifier.urihttp://hdl.handle.net/11449/173781
dc.language.isoeng
dc.relation.ispartofVeterinary Quarterly
dc.relation.ispartofsjr0,520
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCanine
dc.subjectKDNA
dc.subjectLeishmania infantum
dc.subjectPCR
dc.subjectStray dogs
dc.titleDetection of Leishmania (L.) infantum in stray dogs by molecular techniques with sensitive species-specific primersen
dc.typeArtigo
unesp.author.orcid0000-0001-9319-7516[1]

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