Pharmacological evidence that methylene blue inhibits noradrenaline neuronal uptake in the rat vas deferens

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dc.contributor.authorChies, Agnaldo B.
dc.contributor.authorCustódio, Ricardo C.
dc.contributor.authorde Souza, Gregório
dc.contributor.authorCorrêa, Fernando M.A.
dc.contributor.authorPereira, Oduvaldo C.M.
dc.contributor.institutionSchool of Medicine of Marília
dc.contributor.institutionSchool of Medicine of Ribeirao Preto
dc.contributor.institutionBiosciences Institute of Botucatu
dc.date.accessioned2022-04-29T08:42:51Z
dc.date.available2022-04-29T08:42:51Z
dc.date.issued2003-10-09
dc.description.abstractWe report that the classical guanylate cyclase inhibitor methylene blue (MB, 1 μM or 10 μM), but not the selective guanylate cyclase inhibitor 1H-[1,2,4]oxidazolo[4,3-a]quinoxalin-1-one (1 μM) or nitric oxide synthase inhibitor, NG-nitro-L-arginine methyl ester (100 μM), causes a shift to the left in the concentration-response curve for noradrenaline in the isolated rat vas deferens preparations. The main objective of our study was to investigate the pharmacological mechanism by which MB increases the sensitivity of the rat vas deferens to noradrenaline. According to the presented results, MB did not change rat vas deferens sensitivity to methoxamine or noradrenaline in the presence of desipramine (0.1 μM). The pre-contracted rat vas deferens relaxation induced by isoproterenol was also not significantly changed by MB (1 μM). Thus, we suggest that MB increases rat vas deferens sensitivity through neuronal uptake inhibition without interfering in either the nitrergic mechanism or guanylate cyclase activity.en
dc.description.affiliationLaboratory of Pharmacology School of Medicine of Marília, Marília 17519-030 SP
dc.description.affiliationDepartment of Pharmacology School of Medicine of Ribeirao Preto, Ribeirão Preto 14049-900 SP
dc.description.affiliationDepartment of Pharmacology Biosciences Institute of Botucatu, Botucatu 18.618-000 SP
dc.format.extent573-579
dc.identifier.citationPolish Journal of Pharmacology, v. 55, n. 4, p. 573-579, 2003.
dc.identifier.issn1230-6002
dc.identifier.scopus2-s2.0-0141725416
dc.identifier.urihttp://hdl.handle.net/11449/230951
dc.language.isoeng
dc.relation.ispartofPolish Journal of Pharmacology
dc.sourceScopus
dc.subjectGuanylate cyclase
dc.subjectMethylene blue
dc.subjectNeuronal uptake
dc.subjectNoradrenaline
dc.subjectRat vas deferens
dc.titlePharmacological evidence that methylene blue inhibits noradrenaline neuronal uptake in the rat vas deferensen
dc.typeArtigo

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