Adenocarcinoma in females detected in serous effusions: Cytomorphologic aspects and immunocytochemical reactivity to cytokeratins 7 and 20

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dc.contributor.authorLongatto Filho, Adhemar
dc.contributor.authorBisi, Hélio [UNESP]
dc.contributor.authorAlves, Venâncio Avancini Ferreira [UNESP]
dc.contributor.authorKanamura, Cristina Takami
dc.contributor.authorOyafuso, Marina Suheko
dc.contributor.authorBortolan, Jeni
dc.contributor.authorLombardo, Valéria
dc.contributor.institutionInstituto Adolfo Lutz (IAL)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionA. C. Camargo Cancer Center
dc.contributor.institutionOncocentro Foundation
dc.date.accessioned2014-05-27T11:18:14Z
dc.date.available2014-05-27T11:18:14Z
dc.date.issued1997-07-01
dc.description.abstractOBJECTIVE: To investigate the usefulness of assessing the immunoreactivity of cytokeratins 7 (CK7) and 20 (CK20) as well as several cytomorphologic parameters in effusions with metastatic adenocarcinomas in the search for the primary site of the tumor. STUDY DESIGN: From the files of the Pathology Department, A. C. Camargo Hospital, we studied cytologic smears from 73 metastatic adenocarcinomas originally from the breast, 63 from the ovary, 40 from the lung and 32 from the stomach, looking for morphologic parameters that could have discriminant potential in suggesting the primary site in a routine situation, including intranuclear inclusions, prominent nucleoli, mitosis, signet-ring cells, psammoma bodies, nuclear crease, binucleation and multinucleation, papillary features, acinar profile (including ball cells) and single cells. Immunoreactions were performed with monoclonal antibodies to CK7 (OV-TL 12/30 and CK20 (Ks 20.8) and included morphologic analysis. Both analyses were studied in a blind fashion regarding the primary site of the tumors. RESULTS: Positivity ratios for breast, ovary, stomach and lung cases were 67.6%, 63.5%, 29.7% and 45.5%, respectively, for CK7 and 17.2%, 15.8%, 13.5% and 32.2%, respectively, for CK20. Discriminant analysis of morphologic and immunocytochemical parameters had an error rate of 42.9% in recognizing the primary site and a Wilk's lambda of .7290. CONCLUSION: The more efficient parameter with discriminant function was the papillary appearance showed by CK7, which should be used in further studies with a similar scope. The set of parameters used in this study were insufficient to discriminate the primary site of female adenocarcinomas in effusions with significant accuracy.en
dc.description.affiliationPathology Division Adolfo Lutz Institute, São Paulo
dc.description.affiliationPathology Department São Paulo State University Medical School, São Paulo
dc.description.affiliationSurgical Pathology Department A. C. Camargo Hospital, São Paulo
dc.description.affiliationDiv. of Epidemiol. and Biostatistics Oncocentro Foundation, São Paulo
dc.description.affiliationSetor de Citologia Oncotica Divisão de Patologia Instituto Adolfo Lutz, Av. Dr. Arnaldo, 355, São Paulo, SP
dc.description.affiliationUnespPathology Department São Paulo State University Medical School, São Paulo
dc.format.extent961-971
dc.identifierhttp://dx.doi.org/10.1159/000332774
dc.identifier.citationActa Cytologica, v. 41, n. 4, p. 961-971, 1997.
dc.identifier.doi10.1159/000332774
dc.identifier.issn0001-5547
dc.identifier.scopus2-s2.0-0344220153
dc.identifier.urihttp://hdl.handle.net/11449/65136
dc.language.isoeng
dc.relation.ispartofActa Cytologica
dc.relation.ispartofsjr0,480
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAdenocarcinoma
dc.subjectCytokeratin
dc.subjectImmunocytochemistry
dc.subjectcytokeratin
dc.subjectcytokeratin 20
dc.subjectcytokeratin 7
dc.subjectunclassified drug
dc.subjectadenocarcinoma
dc.subjectbreast carcinoma
dc.subjectcancer cytodiagnosis
dc.subjectcancer localization
dc.subjectclinical article
dc.subjecteffusion
dc.subjectfemale
dc.subjecthuman
dc.subjectintermediate filament
dc.subjectlung carcinoma
dc.subjectmetastasis
dc.subjectovary carcinoma
dc.subjectpriority journal
dc.subjectstomach carcinoma
dc.subjectBreast Neoplasms
dc.subjectFemale
dc.subjectHumans
dc.subjectImmunohistochemistry
dc.subjectIntermediate Filament Proteins
dc.subjectKeratin-20
dc.subjectKeratins
dc.subjectLung Neoplasms
dc.subjectNeoplasms, Unknown Primary
dc.subjectOvarian Neoplasms
dc.subjectSerous Membrane
dc.subjectStomach Neoplasms
dc.subjectTumor Markers, Biological
dc.titleAdenocarcinoma in females detected in serous effusions: Cytomorphologic aspects and immunocytochemical reactivity to cytokeratins 7 and 20en
dc.typeArtigo
dcterms.licensehttp://www.karger.com/Services/RightsPermissions
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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