Beneficial Role of Low-Intensity Laser Irradiation on Neural β-tubulin III Protein Expression in Human Bone Marrow Multipotent Mesenchymal Stromal Cells

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dc.contributor.authorFerreira-Silva, Valéria
dc.contributor.authorPrimo, Fernando L. [UNESP]
dc.contributor.authorBaqui, Munira M.A.
dc.contributor.authorMagalhães, Danielle A.R.
dc.contributor.authorOrellana, Maristela D.
dc.contributor.authorCastilho-Fernandes, Andrielle
dc.contributor.authorCruz, Mario C.
dc.contributor.authorCâmara, Niehls O. S.
dc.contributor.authorCovas, Dimas T.
dc.contributor.authorTedesco, Antonio C.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:23:59Z
dc.date.available2018-12-11T17:23:59Z
dc.date.issued2018-08-01
dc.description.abstractThe purpose of the present study was to evaluate the neural protein expression pattern of human multipotent mesenchymal stromal cells (hMSCs) treated with forskolin (free-form/FF). The study investigated forskolin’s capacity to enhance intracellular levels of cyclic adenosine monophosphate (cAMP) by activating adenylate cyclase and probably by inducing neuron-like cells in vitro. In addition, because nanotechnology is a growing field of tissue engineering, we also assessed the action of a new system called the nanostructured-forskolin (NF) to examine the improvement of drug delivery. Afterwards, the cells were submitted to low-level laser irradiation to evaluate possible photobiostimulatory effects. Investigations using the immunofluorescence by confocal microscopy and Western blot methods revealed the expression of the neuronal marker β-tubulin III. Fluorescence intensity quantification analysis using INCell Analyzer System for β-tubulin III was used to examine significant differences. The results showed that after low-level laser irradiation exposure, there was a tendency to increase the β-tubulin III expression in all groups, as expected in the photobiostimulation process. Notably, this process induced for irradiation was more pronounced in irradiated nanoforskolin cells (INF) compared to non-irradiated free-forskolin control cells (NFFC). However, there was also an increase in β-tubulin III protein expression in the groups: irradiated nanocontrol cells (INC) compared to non-irradiated free-forskolin control cells (NFF) and after treatment with non-irradiated free-forskolin (NFF) and non-irradiated nanoforskolin (NNFC). We concluded that the methods using low-level laser irradiation and/or nanoparticles showed an up-regulation of neural-protein expression in hMSCs that could be used to facilitate cellular therapy protocols in the near future.en
dc.description.affiliationDepartment of Chemistry Centre of Nanotechnology and Tissue Engineering-Photobiology and Photomedicine Research Group Faculty of Philosophy Sciences and Letters of Ribeirão Preto University of São Paulo
dc.description.affiliationCentre for Cell Therapy and Regional Blood Centre National Institute of Science and Technology in Stem Cell and Cell Therapy Medical School University of São Paulo
dc.description.affiliationDepartment of Bioprocess and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP)
dc.description.affiliationDepartment of Cell and Molecular Biology and Pathogenic Bioagents Medical School University of São Paulo
dc.description.affiliationInstitute of Biomedical Science Department of Immunology University of São Paulo
dc.description.affiliationDepartment of Clinical Medicine Medical School University of São Paulo
dc.description.affiliationUnespDepartment of Bioprocess and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP)
dc.description.sponsorshipFinanciadora de Estudos e Projetos
dc.description.sponsorshipInstituto Nacional de Ciência e Tecnologia para o Controle das Intoxicações por Plantas
dc.description.sponsorshipIdFinanciadora de Estudos e Projetos: 01.10.0758.01
dc.format.extent585-598
dc.identifierhttp://dx.doi.org/10.1007/s12015-017-9796-3
dc.identifier.citationStem Cell Reviews and Reports, v. 14, n. 4, p. 585-598, 2018.
dc.identifier.doi10.1007/s12015-017-9796-3
dc.identifier.file2-s2.0-85041917888.pdf
dc.identifier.issn1558-6804
dc.identifier.issn1550-8943
dc.identifier.scopus2-s2.0-85041917888
dc.identifier.urihttp://hdl.handle.net/11449/177096
dc.language.isoeng
dc.relation.ispartofStem Cell Reviews and Reports
dc.relation.ispartofsjr1,323
dc.relation.ispartofsjr1,323
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectForskolin
dc.subjectLow-intensity laser irradiation
dc.subjectMultipotent mesenchymal stromal cells
dc.subjectNano-drug delivery
dc.subjectNanotechnology
dc.subjectβ-Tubulin III
dc.titleBeneficial Role of Low-Intensity Laser Irradiation on Neural β-tubulin III Protein Expression in Human Bone Marrow Multipotent Mesenchymal Stromal Cellsen
dc.typeArtigo

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