Antimicrobial photodynamic therapy alone or in combination with antibiotic local administration against biofilms of Fusobacterium nucleatum and Porphyromonas gingivalis

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dc.contributor.authorTavares, Lívia J. [UNESP]
dc.contributor.authorde Avila, Erica D. [UNESP]
dc.contributor.authorKlein, Marlise I. [UNESP]
dc.contributor.authorPanariello, Beatriz H.D.
dc.contributor.authorSpolidório, Denise M.P. [UNESP]
dc.contributor.authorPavarina, Ana Cláudia [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionIndiana University School of Dentistry
dc.date.accessioned2019-10-06T15:57:22Z
dc.date.available2019-10-06T15:57:22Z
dc.date.issued2018-11-01
dc.description.abstractAntimicrobial photodynamic therapy (aPDT) kills several planktonic pathogens. However, the susceptibility of biofilm-derived anaerobic bacteria to aPDT is poorly characterized. Here, we evaluated the effect of Photodithazine (PDZ)-mediated aPDT on Fusobacterium nucleatum and Porphyromonas gingivalis biofilms. In addition, aPDT was tested with metronidazole (MTZ) to explore the potential antimicrobial effect of the treatment. The minimum inhibitory concentration (MIC) of MTZ was defined for each bacterial species. Single-species biofilms of each species were grown on polystyrene plates under anaerobic conditions for five days. aPDT was performed by applying PDZ at concentrations of 50, 75 and 100 mg/L, followed by exposure to 50 J/cm 2 LED light (660 nm) with or without MTZ. aPDT exhibited a significant reduction in bacterial viability at a PDZ concentration of 100 mg/L, with 1.12 log 10 and 2.66 log 10 reductions for F. nucleatum and P. gingivalis in biofilms, respectively. However, the antimicrobial effect against F. nucleatum was achieved only when aPDT was combined with MTZ at 100× MIC. Regarding P. gingivalis, the combination of PDZ-mediated aPDT at 100 mg/L with MTZ 100× MIC resulted in a 5 log 10 reduction in the bacterial population. The potential antimicrobial effects of aPDT in combination with MTZ for both single pathogenic biofilms were confirmed by live/dead staining. These results suggest that localized antibiotic administration may be an adjuvant to aPDT to control F. nucleatum and P. gingivalis biofilms.en
dc.description.affiliationDepartment of Dental Materials and Prosthodontics São Paulo State University (Unesp) School of Dentistry, Araraquara, Rua Humaitá 1680
dc.description.affiliationDepartment of Cariology Operative Dentistry and Dental Public Health Indiana University School of Dentistry, 1121 W Michigan St, DS406
dc.description.affiliationDepartment of Physiology and Pathology São Paulo State University (Unesp) School of Dentistry, Araraquara, Rua Humaitá 1680
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics São Paulo State University (Unesp) School of Dentistry, Araraquara, Rua Humaitá 1680
dc.description.affiliationUnespDepartment of Physiology and Pathology São Paulo State University (Unesp) School of Dentistry, Araraquara, Rua Humaitá 1680
dc.format.extent135-145
dc.identifierhttp://dx.doi.org/10.1016/j.jphotobiol.2018.09.010
dc.identifier.citationJournal of Photochemistry and Photobiology B: Biology, v. 188, p. 135-145.
dc.identifier.doi10.1016/j.jphotobiol.2018.09.010
dc.identifier.issn1873-2682
dc.identifier.issn1011-1344
dc.identifier.scopus2-s2.0-85053823000
dc.identifier.urihttp://hdl.handle.net/11449/188102
dc.language.isoeng
dc.relation.ispartofJournal of Photochemistry and Photobiology B: Biology
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAnaerobic bacteria
dc.subjectCombined modality therapy
dc.subjectMetronidazole
dc.subjectPhotodynamic therapy
dc.titleAntimicrobial photodynamic therapy alone or in combination with antibiotic local administration against biofilms of Fusobacterium nucleatum and Porphyromonas gingivalisen
dc.typeArtigo
unesp.author.lattes8867670539105403[6]
unesp.author.orcid0000-0002-9231-1994[6]

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Artigos - Materiais Odontológicos e Prótese - FOAR
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