Siloxane-modified bacterial cellulose as a promising platform for cell culture

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dc.contributor.authorClaro, Amanda Maria
dc.contributor.authorDo Amaral, Nayara Cavichiolli
dc.contributor.authorColturato, Vitória Maria Medalha
dc.contributor.authorAleixo, Nadia Andrade
dc.contributor.authorPaiva, Robert
dc.contributor.authorCruz, Sandra Andrea
dc.contributor.authorMonteiro, Gustavo Claro
dc.contributor.authorDe Carvalho, Gustavo Senra Gonçalves
dc.contributor.authorNogueira, Flávia Aparecida Resende
dc.contributor.authorDeffune, Elenice [UNESP]
dc.contributor.authorIemma, Mônica Rosas da Costa
dc.contributor.authorBarud, Hernane da Silva
dc.contributor.institutionUniversity of Araraquara - UNIARA
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionTechMiP Análises e Soluções Inteligentes LTDA
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T14:52:48Z
dc.date.available2023-07-29T14:52:48Z
dc.date.issued2022-12-01
dc.description.abstractCellulose is a versatile tunable material that finds application in the biomedical field as substrate for cell culture. However, few studies have been done to explore the use of bacterial cellulose (BC), a naturally occurring nanofibrillar material with distinct properties, as a platform to produce such device. In the present work, BC membranes have been functionalized with thiol functional group (SH) through silanization reaction with (3-mercaptopropyl)trimethoxysilane (MPTMS) under different conditions, in order to obtain a platform with improved cell adhesion. The efficiency of BC surface modification with MPTMS was evaluated by using acid and base catalyzed reactions, and two different drying methods: at room temperature of 28 °C and at 120 °C as curing temperature. The results of the set of analyses performed—ATR-FTIR, TGA, elemental analysis,13C NMR, contact angle and SEM—indicate that BC surface functionalization was efficient, regardless the drying process. The MPTMS-modified platforms exhibited sulfur content of 3–5 times higher than native BC. The performed biological assay with fibroblast GM07492 human cells revealed that adhesion of cells to the BC surface depends not only on the functional group present at the matrix but also on surface wettability. Graphical abstract: [Figure not available: see fulltext.]Schematic illustration of a siloxane-modified bacterial cellulose as platform for cell culture.en
dc.description.affiliationUniversity of Araraquara - UNIARA, Rua Carlos Gomes 1217, SP
dc.description.affiliationDepartment of Chemistry Federal University of São Carlos, P.O. Box 676, SP
dc.description.affiliationTechMiP Análises e Soluções Inteligentes LTDA, Av. Jorge Fernandes de Mattos 311, SP
dc.description.affiliationMedical School Sao Paulo State University Julio de Mesquita Filho, Av. Prof. Mário Rubens Guimarães Montenegro, s/n - UNESP - Campus de Botucatu
dc.description.affiliationUnespMedical School Sao Paulo State University Julio de Mesquita Filho, Av. Prof. Mário Rubens Guimarães Montenegro, s/n - UNESP - Campus de Botucatu
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2018/23853-2
dc.description.sponsorshipIdFAPESP: 2019/12711-5
dc.description.sponsorshipIdFAPESP: 2020/05163-9
dc.format.extent9597-9608
dc.identifierhttp://dx.doi.org/10.1007/s10570-022-04872-4
dc.identifier.citationCellulose, v. 29, n. 18, p. 9597-9608, 2022.
dc.identifier.doi10.1007/s10570-022-04872-4
dc.identifier.issn1572-882X
dc.identifier.issn0969-0239
dc.identifier.scopus2-s2.0-85140061405
dc.identifier.urihttp://hdl.handle.net/11449/249277
dc.language.isoeng
dc.relation.ispartofCellulose
dc.sourceScopus
dc.subjectBacterial cellulose
dc.subjectCell culture
dc.subjectMercapto
dc.subjectPlatform
dc.subjectSiloxane
dc.titleSiloxane-modified bacterial cellulose as a promising platform for cell cultureen
dc.typeArtigo
unesp.author.orcid0000-0003-0076-7298[1]
unesp.author.orcid0000-0003-3994-0916[2]
unesp.author.orcid0000-0002-2246-1057[3]
unesp.author.orcid0000-0003-3717-0671[4]
unesp.author.orcid0000-0001-5010-8173[5]
unesp.author.orcid0000-0002-5548-0166[6]
unesp.author.orcid0000-0002-3011-3028[7]
unesp.author.orcid0000-0003-1632-8070[8]
unesp.author.orcid0000-0002-9432-5919[9]
unesp.author.orcid0000-0002-0533-3248[10]
unesp.author.orcid0000-0003-1173-2111[11]
unesp.author.orcid0000-0001-9081-2413[12]

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