Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samples

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dc.contributor.authorDel Vecchio, Adriana [UNESP]
dc.contributor.authorSilva, Julhiany de Fatima da [UNESP]
dc.contributor.authorSilva, Juliana Leal Monteiro da [UNESP]
dc.contributor.authorAndreotti, Patricia Ferrari [UNESP]
dc.contributor.authorSoares, Christiane Pienna [UNESP]
dc.contributor.authorBenard, Gil
dc.contributor.authorMendes-Giannini, Maria José Soares [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:23:46Z
dc.date.available2014-05-20T13:23:46Z
dc.date.issued2009-08-01
dc.description.abstractParacoccidioidomycosis presents a variety of clinical manifestations and Paracoccidioides brasiliensis can reach many tissues, most importantly the lungs. The ability of the pathogen to interact with host surface structures is essential to its virulence. The interaction between P. brasiliensis and epithelial cells has been studied, with particular emphasis on the induction of apoptosis. To investigate the expression of different apoptosis-inducing pathways in human A549 cells, we infected these cells with P. brasiliensis Pb18SP (subcultured) and 18R (recently isolated from cell culture and showing a high adhesion pattern) samples in vitro. The expressions of Bcl-2, Bak and caspase 3 were analysed by flow cytometry and DNA fragmentation using the TUNEL technique. Apoptosis of human A549 cells was induced by P. brasiliensis in a sample and time-dependent manner. Using an in vitro model, our data demonstrates that caspase 3, Bak, Bcl-2 and DNA fragmentation mediate P. brasiliensis-induced apoptosis in A549 cells. The overall mechanism is a complex process, which may involve several signal transduction pathways. These findings could partially explain the efficient behaviour of this fungus in promoting tissue infection and/or blood dissemination.en
dc.description.affiliationUniversidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Análises Clínicas
dc.description.affiliationInstituto de Medicina Tropical Laboratório de Investigação Médica
dc.description.affiliationUniversidade de São Paulo Faculdade de Medicina Hospital das Clínicas
dc.description.affiliationUnespUniversidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Análises Clínicas
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent749-754
dc.identifierhttp://dx.doi.org/10.1590/S0074-02762009000500015
dc.identifier.citationMemórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 104, n. 5, p. 749-754, 2009.
dc.identifier.doi10.1590/S0074-02762009000500015
dc.identifier.fileS0074-02762009000500015.pdf
dc.identifier.issn0074-0276
dc.identifier.lattes1768025290373669
dc.identifier.orcid0000-0002-8059-0826
dc.identifier.orcid0000-0003-1740-7360
dc.identifier.scieloS0074-02762009000500015
dc.identifier.urihttp://hdl.handle.net/11449/7231
dc.identifier.wosWOS:000270684800015
dc.language.isoeng
dc.publisherInstituto Oswaldo Cruz, Ministério da Saúde
dc.relation.ispartofMemórias do Instituto Oswaldo Cruz
dc.relation.ispartofjcr2.833
dc.relation.ispartofsjr1,172
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectapoptosisen
dc.subjectParacoccidioides brasiliensisen
dc.subjectcaspase 3en
dc.subjectBaken
dc.subjectBcl-2en
dc.titleInduction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samplesen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/mioc/paboutj.htm
unesp.author.lattes1768025290373669[5]
unesp.author.orcid0000-0002-8059-0826[7]
unesp.author.orcid0000-0003-1740-7360[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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Artigos - Análises Clínicas - FCFAR